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| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 04383nam a2200613 4500 | |
| 001 | oai:DiVA.org:kth-11446 | |
| 003 | SwePub | |
| 008 | 091110s2009 | |||||||||||000 ||eng| | |
| 020 | a 9789174154900q print | |
| 024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-114462 URI |
| 040 | a (SwePub)kth | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a vet2 swepub-contenttype |
| 072 | 7 | a dok2 swepub-publicationtype |
| 100 | 1 | a Klevebring, Daniel,d 1981-u KTH,Genteknologi,Division of Gene Technology4 aut0 (Swepub:kth)u10ab3gt |
| 245 | 1 0 | a On Transcriptome Sequencing |
| 264 | 1 | a Stockholm :b KTH,c 2009 |
| 300 | a 52 s. | |
| 338 | a electronic2 rdacarrier | |
| 490 | 0 | a Trita-BIO-Report,x 1654-2312 ;v 2009:26 |
| 500 | a QC 20100723 | |
| 520 | a This thesis is about the use of massive DNA sequencing to investigate the transcriptome. During recent decades, several studies have made it clear that the transcriptome comprises a more complex set of biochemical machinery than was previously believed. The majority of the genome can be expressed as transcripts; and overlapping and antisense transcription is widespread. New technologies for the interroga- tion of nucleic acids have made it possible to investigate such cellular phenomena in much greater detail than ever before. For each application, special requirements need to be met. The work presented in this thesis focuses on the transcrip- tome and the development of technology for its analysis. In paper I, we report our development of an automated approach for sample preparation. The procedure was benchmarked against a publicly available reference data set, and we note that our approach outperformed similar manual procedures in terms of reproducibility. In the work reported in papers II-IV, we used different massive sequencing technologies to investigate the transcriptome. In paper II we describe a concatemerization approach that increased throughput by 65% using 454 sequencing,and we identify classes of transcripts not previously described in Populus. Papers III and IV both report studies based on SOLiD sequencing. In the former, we investigated transcripts and proteins for 13% of the human gene and detected a massive overlap for the upper 50% transcriptional levels. In the work described in paper IV, we investigated transcription in non-genic regions of the genome and detected expression from a high number of previ- ously unknown loci. | |
| 650 | 7 | a NATURVETENSKAPx Biologix Annan biologi0 (SwePub)106992 hsv//swe |
| 650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Other Biological Topics0 (SwePub)106992 hsv//eng |
| 650 | 7 | a NATURVETENSKAPx Biologix Bioinformatik och systembiologi0 (SwePub)106102 hsv//swe |
| 650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Bioinformatics and Systems Biology0 (SwePub)106102 hsv//eng |
| 650 | 7 | a NATURVETENSKAPx Biologix Genetik0 (SwePub)106092 hsv//swe |
| 650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Genetics0 (SwePub)106092 hsv//eng |
| 650 | 7 | a NATURVETENSKAPx Biologix Biokemi och molekylärbiologi0 (SwePub)106022 hsv//swe |
| 650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Biochemistry and Molecular Biology0 (SwePub)106022 hsv//eng |
| 650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Cell- och molekylärbiologi0 (SwePub)301082 hsv//swe |
| 650 | 7 | a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Cell and Molecular Biology0 (SwePub)301082 hsv//eng |
| 653 | a Transcriptome | |
| 653 | a RNA-seq | |
| 653 | a DNA sequencing | |
| 653 | a gene expression profiling | |
| 653 | a non-coding RNA | |
| 653 | a small RNA | |
| 653 | a Functional genomics | |
| 653 | a Funktionsgenomik | |
| 653 | a Bioinformatics | |
| 653 | a Bioinformatik | |
| 653 | a Genetics | |
| 653 | a Genetik | |
| 653 | a Molecular biology | |
| 653 | a Molekylärbiologi | |
| 653 | a Cell biology | |
| 653 | a Cellbiologi | |
| 700 | 1 | a Lundeberg, Joakim,c Professoru KTH,Genteknologi4 ths0 (Swepub:kth)u1qkn9kw |
| 700 | 1 | a Gyllensten, Ulf,c Professoru Uppsala Universitet, Institutionen för genetik och patologi4 opn |
| 710 | 2 | a KTHb Genteknologi4 org |
| 856 | 4 | u https://kth.diva-portal.org/smash/get/diva2:278576/FULLTEXT01.pdfx primaryx Raw objecty fulltext |
| 856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-11446 |
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