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Multi-level characterization of balanced inhibitory-excitatory cortical neuron network derived from human pluripotent stem cells
- Nadadhur, A. G. (author)
- Melero, J. E. (author)
- Meijer, M. (author)
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- Schut, D. (author)
- Jacobs, G. (author)
- Wan Li, K. (author)
- Meredith, R. M. (author)
- Toonen, R. F. (author)
- Van Kesteren, R. E. (author)
- Smit, A. B. (author)
- Verhage, M. (author)
- Heine, V. M. (author)
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- (creator_code:org_t)
- 2017-06-06
- 2017
- English.
- In: PLOS ONE. - : Public Library of Science. - 1932-6203. ; 12:6
- Journal article (peer-reviewed)
Abstract
Subject headings
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- Generation of neuronal cultures from induced pluripotent stem cells (hiPSCs) serve the studies of human brain disorders. However we lack neuronal networks with balanced excitatory- inhibitory activities, which are suitable for single cell analysis. We generated low-density networks of hPSC-derived GABAergic and glutamatergic cortical neurons. We used two different co-culture models with astrocytes. We show that these cultures have balanced excitatory-inhibitory synaptic identities using confocal microscopy, electrophysiological recordings, calcium imaging and mRNA analysis. These simple and robust protocols offer the opportunity for single-cell to multi-level analysis of patient hiPSC-derived cortical excitatory- inhibitory networks; thereby creating advanced tools to study disease mechanisms underlying neurodevelopmental disorders.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- Ganglionic Eminence
- Astrocyte Coculture
- Differentiation
- Interneurons
- Brain
- Gaba
- Specification
- Maturation
- Glutamate
- Disease
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- ref (subject category)
- art (subject category)
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- Nadadhur, A. G.
- Melero, J. E.
- Meijer, M.
- Schut, D.
- Jacobs, G.
- Wan Li, K.
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- Hjorth, Johannes
- Meredith, R. M.
- Toonen, R. F.
- Van Kesteren, R. ...
- Smit, A. B.
- Verhage, M.
- Heine, V. M.
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- MEDICAL AND HEALTH SCIENCES
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- PLOS ONE
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- Royal Institute of Technology
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