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Development of a ty...
Development of a tyrosine-tyrosinase it for connecting cellular communication with electronic networks
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VanArsdale, Eric (författare)
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- Hörnström, David (författare)
- KTH,Industriell bioteknologi
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- Sjöberg, Gustav (författare)
- KTH,Industriell bioteknologi
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visa fler...
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Järbur, Ida (författare)
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Payne, Gregory F. (författare)
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- Van Maris, Antonius J. (författare)
- KTH,Industriell bioteknologi
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Bentley, William E. (författare)
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visa färre...
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(creator_code:org_t)
- Engelska.
- Relaterad länk:
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https://urn.kb.se/re...
Abstract
Ämnesord
Stäng
- There is a growing interest in mediating information transfer between biology and electronics. By the addition of redox mediators to various samples, one can electronically obtain a redox “portrait” of a biological system and additionally program gene expression in suitably engineered cells. We have created a cell-based synthetic biology-electrochemical axis in which engineered cells process molecular cues producing an output that can be directly recorded via electronics – without added redox mediators. The process is robust; two key components must be together to provide a valid signal. The system builds on the tyrosinase-mediated conversion of tyrosine to L-DOPA and L-DOPAquinone, which are both redox active. “Sensor” cells provide for signal-mediated surface expression of tyrosinase. Similarly, “producer” cells synthesize and export tyrosine. In co-cultures, this system enables real-time electrochemical transduction of the original molecular cues. To demonstrate, we eavesdrop on the quorum sensing molecules from Pseudomonas aeruginosa N-(3-oxododecanoyl)-l-homoserine lactone and pyocyanin.
Ämnesord
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology (hsv//eng)
Nyckelord
- Biotechnology
- Bioteknologi
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