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Pushing the Resolut...
Pushing the Resolution Limit of Stimulated Emission Depletion Optical Nanoscopy
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- Jeong, Sejoo (författare)
- Department of New Biology, DGIST, Daegu, 42988, Republic of Korea, Daegu
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- Koh, Dongbin (författare)
- School of Undergraduate Studies, DGIST, Daegu, 42988, Republic of Korea, Daegu
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- Gwak, Eunha (författare)
- Department of New Biology, DGIST, Daegu, 42988, Republic of Korea, Daegu
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- Venugopal Srambickal, Chinmaya (författare)
- KTH,Kvant- och biofotonik
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- Seo, Daeha (författare)
- Department of Physics and Chemistry, DGIST, Daegu, 42988, Republic of Korea, Daegu
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- Widengren, Jerker (författare)
- KTH,Kvant- och biofotonik
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- Lee, Jong Chan (författare)
- Department of New Biology, DGIST, Daegu, 42988, Republic of Korea, Daegu; New Biology Research Center, DGIST, Daegu, 42988, Republic of Korea, Daegu
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(creator_code:org_t)
- MDPI AG, 2024
- 2024
- Engelska.
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Ingår i: International Journal of Molecular Sciences. - : MDPI AG. - 1661-6596 .- 1422-0067. ; 25:1
- Relaterad länk:
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https://doi.org/10.3...
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https://urn.kb.se/re...
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https://doi.org/10.3...
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Abstract
Ämnesord
Stäng
- Optical nanoscopy, also known as super-resolution optical microscopy, has provided scientists with the means to surpass the diffraction limit of light microscopy and attain new insights into nanoscopic structures and processes that were previously inaccessible. In recent decades, numerous studies have endeavored to enhance super-resolution microscopy in terms of its spatial (lateral) resolution, axial resolution, and temporal resolution. In this review, we discuss recent efforts to push the resolution limit of stimulated emission depletion (STED) optical nanoscopy across multiple dimensions, including lateral resolution, axial resolution, temporal resolution, and labeling precision. We introduce promising techniques and methodologies building on the STED concept that have emerged in the field, such as MINSTED, isotropic STED, and event-triggered STED, and evaluate their respective strengths and limitations. Moreover, we discuss trade-off relationships that exist in far-field optical microscopy and how they come about in STED optical nanoscopy. By examining the latest developments addressing these aspects, we aim to provide an updated overview of the current state of STED nanoscopy and its potential for future research.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biofysik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biophysics (hsv//eng)
- NATURVETENSKAP -- Fysik -- Atom- och molekylfysik och optik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences -- Atom and Molecular Physics and Optics (hsv//eng)
- NATURVETENSKAP -- Fysik -- Annan fysik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences -- Other Physics Topics (hsv//eng)
Nyckelord
- adaptive illumination
- fluorescence lifetime
- fluorescent protein
- fluorophore localization
- nanobody
- optical nanoscopy
- resolution
- STED
- super-resolution microscopy
- temporal resolution
Publikations- och innehållstyp
- ref (ämneskategori)
- for (ämneskategori)
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