Sequence analysis of a partial HbSERK gene expressed during somatic embryo initiation in Hevea brasiliensis

Jayasree, KumariRubber Research Institute of India, Kottayam-686 009, Kerala, India (författare)

QC 20240703
Somatic Embryogenesis Receptor Kinase (SERK) genes are known to express during the transition of somatic cells to embryogenic cells, which have the capability to differentiate into somatic embryos at a later stage of development. In the past 20 years, SERK have drawn much research interest due to its crucial role in somatic embryogenesis. The aim of the current study was to isolate and characterize HbSERK gene through cDNA cloning and to perform comparative genomic analysis. The partial cDNA sequence of HbSERK was 408 bp in length and comparison with genomic sequence revealed the presence of two exons separated by one intron. In silico analysis of HbSERK predicted a protein of 136 amino acids that revealed high similarity (99%) to amino acid of AtSERK1. Multiple sequence alignment showed that HbSERK possessed partial sequences from the characteristic intracellular kinase domain containing the subdomains from V-X of the SERK family. Molecular phylogeny of HbSERK with SERK from other plant species showed that HbSERK was clustered most closely with AtSERK1 and MtSERK1.

Mathew, Suni AnieRubber Research Institute of India, Kottayam-686 009, Kerala, India (författare)
Sivan, Pramod,1984-Rubber Research Institute of India, Kottayam-686 009, Kerala, India(Swepub:kth)u1krnrmy (författare)
K R, SarimolRubber Research Institute of India, Kottayam-686 009, Kerala, India (författare)
Rubber Research Institute of India, Kottayam-686 009, Kerala, India (creator_code:org_t)

Ingår i:Rubber ScienceIndia : Rubber Research Institute of India34:2, s. 145-1542454-48412454-485X

Av författaren/redakt...: Jayasree, Kumari; Mathew, Suni Ani ...; Sivan, Pramod, 1 ...; K R, Sarimol

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