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Sökning: onr:"swepub:oai:DiVA.org:liu-120047" > Effect of Storage T...

LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00004089naa a2200421 4500
001oai:DiVA.org:liu-120047
003SwePub
008150706s2015 | |||||||||||000 ||eng|
0247 a https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-1200472 URI
0247 a https://doi.org/10.1371/journal.pone.01283062 DOI
040 a (SwePub)liu
041 a engb eng
042 9 SwePub
072 7a ref2 swepub-contenttype
072 7a art2 swepub-publicationtype
1001 a Islam, Rakibulu University of Oslo, Norway; Oslo University Hospital, Norway; Harvard University, MA USA4 aut
2451 0a Effect of Storage Temperature on Structure and Function of Cultured Human Oral Keratinocytes
264 c 2015-06-08
264 1b Public Library of Science,c 2015
338 a electronic2 rdacarrier
500 a Funding Agencies|Department of Oral Biology, Faculty of Dentistry, University of Oslo, Oslo, Norway [5142001]
520 a Purpose/Aims To assess the effect of storage temperature on the viability, phenotype, metabolism, and morphology of cultured human oral keratinocytes (HOK). Materials and Methods Cultured HOK cells were stored in HEPES- and sodium bicarbonate-buffered Minimum Essential Medium (MEM) at nine temperatures in approximately 4 degrees C increments from 4 degrees C to 37 degrees C for seven days. Cells were characterized for viability by calcein fluorescence, phenotype retention by immunocytochemistry, metabolic parameters (pH, glucose, lactate, and O-2) within the storage medium by blood gas analysis, and morphology by scanning electron microscopy and light microscopy. Results Relative to the cultured, but non-stored control cells, a high percentage of viable cells were retained only in the 12 degrees C and 16 degrees C storage groups (85%+/- 13% and 68%+/- 10%, respectively). Expression of ABCG2, Bmi1, C/EBP delta, PCNA, cytokeratin 18, and caspase-3 were preserved after storage in the 5 groups between 4 degrees C and 20 degrees C, compared to the non-stored control. Glucose, pH and pO(2) in the storage medium declined, whereas lactate increased with increasing storage temperature. Morphology was best preserved following storage of the three groups between 12 degrees C, 16 degrees C, and 20 degrees C. Conclusion We conclude that storage temperatures of 12 degrees C and 16 degrees C were optimal for maintenance of cell viability, phenotype, and morphology of cultured HOK. The storage method described in the present study may be applicable for other cell types and tissues; thus its significance may extend beyond HOK and the field of ophthalmology.
650 7a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicin0 (SwePub)3022 hsv//swe
650 7a MEDICAL AND HEALTH SCIENCESx Clinical Medicine0 (SwePub)3022 hsv//eng
7001 a Jackson, Catherineu Oslo University Hospital, Norway4 aut
7001 a Eidet, Jon R.u Oslo University Hospital, Norway4 aut
7001 a Messelt, Edward B.u University of Oslo, Norway4 aut
7001 a Maria Corraya, Rimau Oslo University Hospital, Norway; Harvard University, MA USA4 aut
7001 a Lyberg, Torsteinu Oslo University Hospital, Norway4 aut
7001 a Griffith, Mayu Linköpings universitet,Avdelningen för cellbiologi,Medicinska fakulteten4 aut0 (Swepub:liu)maygr76
7001 a Dartt, Darlene A.u Harvard University, MA USA4 aut
7001 a Utheim, Tor P.u University of Oslo, Norway; Oslo University Hospital, Norway; Harvard University, MA USA4 aut
7102 a University of Oslo, Norway; Oslo University Hospital, Norway; Harvard University, MA USAb Oslo University Hospital, Norway4 org
7730 t PLOS ONEd : Public Library of Scienceg 10:6, s. e0128306-q 10:6<e0128306-x 1932-6203
8564 u https://liu.diva-portal.org/smash/get/diva2:840007/FULLTEXT01.pdfx primaryx Raw objecty fulltext:print
8564 u https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0128306&type=printable
8564 8u https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-120047
8564 8u https://doi.org/10.1371/journal.pone.0128306

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