Sökning: onr:"swepub:oai:DiVA.org:liu-148383" >
Codon optimization ...
Codon optimization and improved delivery/immunization regimen enhance the immune response against wild-type and drug-resistant HIV-1 reverse transcriptase, preserving its Th2-polarity
-
- Latanova, A. A. (författare)
- Russian Acad Sci, Russia; Karolinska Inst, Sweden; Gamaleja Res Ctr Epidemiol and Microbiol, Russia
-
- Petkov, S. (författare)
- Karolinska Institutet,Karolinska Inst, Sweden
-
- Kilpelainen, A. (författare)
- Karolinska Inst, Sweden
-
visa fler...
-
- Jansons, J. (författare)
- Riga Stradins Univ, Latvia
-
- Latyshev, O. E. (författare)
- Gamaleja Res Ctr Epidemiol and Microbiol, Russia; Russian Acad Sci, Russia
-
- Kuzmenko, Y. V. (författare)
- Russian Acad Sci, Russia
-
- Hinkula, Jorma (författare)
- Linköpings universitet,Avdelningen för mikrobiologi och molekylär medicin,Medicinska fakulteten,Karolinska Inst, Sweden
-
- Abakumov, M. A. (författare)
- Pirogov Russian Natl Res Med Univ, Russia; Natl Univ Sci and Technol MISIS, Russia
-
- Valuev-Elliston, V. T. (författare)
- Russian Acad Sci, Russia
-
- Gomelsky, M. (författare)
- Karolinska Institutet,Univ Wyoming, WY 82071 USA,Gamaleja Res Ctr Epidemiol and Microbiol, Russia; Russian Acad Sci, Russia; Riga Stradins Univ, Latvia
-
- Karpov, V. L. (författare)
- Russian Acad Sci, Russia
-
- Chiodi, F. (författare)
- Karolinska Institutet,Karolinska Inst, Sweden
-
- Wahren, B. (författare)
- Karolinska Institutet,Karolinska Inst, Sweden
-
- Logunov, D. Y. (författare)
- Gamaleja Res Ctr Epidemiol and Microbiol, Russia; Russian Acad Sci, Russia
-
- Starodubova, E. S. (författare)
- Russian Acad Sci, Russia
-
- Isaguliants, M. G. (författare)
- Gamaleja Res Ctr Epidemiol and Microbiol, Russia; Russian Acad Sci, Russia; Riga Stradins Univ, Latvia
-
visa färre...
-
(creator_code:org_t)
- 2018-05-24
- 2018
- Engelska.
-
Ingår i: Scientific Reports. - : NATURE PUBLISHING GROUP. - 2045-2322. ; 8
- Relaterad länk:
-
https://liu.diva-por... (primary) (Raw object)
-
visa fler...
-
https://www.nature.c...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
http://kipublication...
-
visa färre...
Abstract
Ämnesord
Stäng
- DNA vaccines require a considerable enhancement of immunogenicity. Here, we optimized a prototype DNA vaccine against drug-resistant HIV-1 based on a weak Th2-immunogen, HIV-1 reverse transcriptase (RT). We designed expression-optimized genes encoding inactivated wild-type and drug-resistant RTs (RT-DNAs) and introduced them into mice by intradermal injections followed by electroporation. RT-DNAs were administered as single or double primes with or without cyclic-di-GMP, or as a prime followed by boost with RT-DNA mixed with a luciferase-encoding plasmid ("surrogate challenge"). Repeated primes improved cellular responses and broadened epitope specificity. Addition of cyclic-di-GMP induced a transient increase in IFN-gamma production. The strongest anti-RT immune response was achieved in a prime-boost protocol with electroporation by short 100V pulses done using penetrating electrodes. The RT-specific response, dominated by CD4+T-cells, targeted epitopes at aa 199-220 and aa 528-543. Drug-resistance mutations disrupted the epitope at aa 205-220, while the CTL epitope at aa 202-210 was not affected. Overall, multiparametric optimization of RT strengthened its Th2- performance. A rapid loss of RT/luciferase-expressing cells in the surrogate challenge experiment revealed a lytic potential of anti-RT response. Such lytic CD4+ response would be beneficial for an HIV vaccine due to its comparative insensitivity to immune escape.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Latanova, A. A.
-
Petkov, S.
-
Kilpelainen, A.
-
Jansons, J.
-
Latyshev, O. E.
-
Kuzmenko, Y. V.
-
visa fler...
-
Hinkula, Jorma
-
Abakumov, M. A.
-
Valuev-Elliston, ...
-
Gomelsky, M.
-
Karpov, V. L.
-
Chiodi, F.
-
Wahren, B.
-
Logunov, D. Y.
-
Starodubova, E. ...
-
Isaguliants, M. ...
-
visa färre...
- Om ämnet
-
- MEDICIN OCH HÄLSOVETENSKAP
-
MEDICIN OCH HÄLS ...
-
och Medicinska och f ...
-
och Mikrobiologi ino ...
- Artiklar i publikationen
-
Scientific Repor ...
- Av lärosätet
-
Linköpings universitet
-
Karolinska Institutet