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LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00003352naa a2200421 4500
001oai:DiVA.org:liu-161618
003SwePub
008191105s2019 | |||||||||||000 ||eng|
0247 a https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-1616182 URI
0247 a https://doi.org/10.1111/rda.135192 DOI
040 a (SwePub)liu
041 a engb eng
042 9 SwePub
072 7a ref2 swepub-contenttype
072 7a art2 swepub-publicationtype
1001 a Peris-Frau, Patriciau SaBio IREC CSIC UCLM JCCM, Spain4 aut
2451 0a Comparative evaluation of DNA integrity using sperm chromatin structure assay and Sperm-Ovis-Halomax during in vitro capacitation of cryopreserved ram spermatozoa
264 c 2019-10-17
264 1b WILEY,c 2019
338 a electronic2 rdacarrier
500 a Funding Agencies|ALF-Research, Region Ostergotland [LIO-698951]; Lions Forskningsfond [DNR LIU-2016-00641]; Linkoping and the Swedish Research Council FormasSwedish Research Council Formas [2017-00946]; Forskningsradet i Sydostra Sverige [473121, 745971]; Universidad de Castilla-La Mancha [2015/4062]
520 a This study aimed to compare the ability of sperm chromatin structure assay (SCSA (R)) and Sperm-Ovis-Halomax (R) to detect DNA fragmentation in frozen-thawed ram spermatozoa incubated under capacitating conditions in synthetic oviductal fluid (SOF) supplemented with oestrous sheep serum (SOF-ESS) at multiple time points (0-240 min). Incubation in SOF-ESS had no significant effects on SCSA (R) parameters while the percentage of spermatozoa with fragmented DNA measured by Sperm-Ovis-Halomax (R) increased after 180 min of incubation. In addition, no correlation or agreement was found between the techniques, suggesting that SCSA (R) and Sperm-Ovis-Halomax (R) may quantify different types of DNA damage in ram spermatozoa under these experimental conditions.
650 7a NATURVETENSKAPx Biologix Biofysik0 (SwePub)106032 hsv//swe
650 7a NATURAL SCIENCESx Biological Sciencesx Biophysics0 (SwePub)106032 hsv//eng
653 a DNA integrity; sperm capacitation; SCSA (R); Sperm-Ovis-Halomax (R)
7001 a Alvarez-Rodriguez, Manuelu Linköpings universitet,Avdelningen för barns och kvinnors hälsa,Medicinska fakulteten4 aut0 (Swepub:liu)manal80
7001 a Martin-Maestro, Aliciau SaBio IREC CSIC UCLM JCCM, Spain4 aut
7001 a Iniesta-Cuerda, Mariau SaBio IREC CSIC UCLM JCCM, Spain4 aut
7001 a Sanchez-Ajofrin, Ireneu SaBio IREC CSIC UCLM JCCM, Spain4 aut
7001 a Julian Garde, Joseu SaBio IREC CSIC UCLM JCCM, Spain4 aut
7001 a Rodriguez-Martinez, Heribertou Linköpings universitet,Avdelningen för barns och kvinnors hälsa,Medicinska fakulteten4 aut0 (Swepub:liu)herro55
7001 a Josefa Soler, Anau SaBio IREC CSIC UCLM JCCM, Spain4 aut
7102 a SaBio IREC CSIC UCLM JCCM, Spainb Avdelningen för barns och kvinnors hälsa4 org
7730 t Reproduction in domestic animalsd : WILEYg 54, s. 46-49q 54<46-49x 0936-6768x 1439-0531
8564 u https://liu.diva-portal.org/smash/get/diva2:1367907/FULLTEXT01.pdfx primaryx Raw objecty fulltext:postprint
8564 u http://liu.diva-portal.org/smash/get/diva2:1367907/FULLTEXT01
8564 8u https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-161618
8564 8u https://doi.org/10.1111/rda.13519

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