onr:"swepub:oai:DiVA.org:liu-199533"
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| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 04336naa a2200361 4500 | |
| 001 | oai:DiVA.org:liu-199533 | |
| 003 | SwePub | |
| 008 | 231211s2024 | |||||||||||000 ||eng| | |
| 024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-1995332 URI |
| 024 | 7 | a https://doi.org/10.1096/fj.202301520R2 DOI |
| 040 | a (SwePub)liu | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a ref2 swepub-contenttype |
| 072 | 7 | a art2 swepub-publicationtype |
| 100 | 1 | a Bratengeier, Corneliau Linköpings universitet,Avdelningen för cellbiologi,Medicinska fakulteten4 aut0 (Swepub:liu)corbr78 |
| 245 | 1 0 | a Mechanical loading intensities affect the release of extracellular vesicles from mouse bone marrow-derived hematopoietic progenitor cells and change their osteoclast-modulating effect |
| 264 | 1 | b WILEY,c 2024 |
| 338 | a print2 rdacarrier | |
| 500 | a Funding Agencies|Region Ostergotland (Ostergotland Region); Vetenskapsradet (VR) [2016-01822, 2019-01016, 2019-03636] | |
| 520 | a Low-intensity loading maintains or increases bone mass, whereas lack of mechanical loading and high-intensity loading decreases bone mass, possibly via the release of extracellular vesicles by mechanosensitive bone cells. How different loading intensities alter the biological effect of these vesicles is not fully understood. Dynamic fluid shear stress at low intensity (0.7 +/- 0.3 Pa, 5 Hz) or high intensity (2.9 +/- 0.2 Pa, 1 Hz) was used on mouse hematopoietic progenitor cells for 2 min in the presence or absence of chemical compounds that inhibit release or biogenesis of extracellular vesicles. We used a Receptor activator of nuclear factor kappa-Beta ligand-induced osteoclastogenesis assay to evaluate the biological effect of different fractions of extracellular vesicles obtained through centrifugation of medium from hematopoietic stem cells. Osteoclast formation was reduced by microvesicles (10 000x g) obtained after low-intensity loading and induced by exosomes (100 000x g) obtained after high-intensity loading. These osteoclast-modulating effects could be diminished or eliminated by depletion of extracellular vesicles from the conditioned medium, inhibition of general extracellular vesicle release, inhibition of microvesicle biogenesis (low intensity), inhibition of ESCRT-independent exosome biogenesis (high intensity), as well as by inhibition of dynamin-dependent vesicle uptake in osteoclast progenitor cells. Taken together, the intensity of mechanical loading affects the release of extracellular vesicles and change their osteoclast-modulating effect. The intensity of mechanical loading strongly affects bone remodeling by either formation of bone or resorption of bone. Low-intensity loading on bone cells releases microvesicles that reduce formation of bone-resorbing osteoclasts, while high-intensity loading on bone cells releases exosomes that induce formation of bone-resorbing osteoclasts. The graphical abstract was created with image | |
| 650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Cell- och molekylärbiologi0 (SwePub)301082 hsv//swe |
| 650 | 7 | a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Cell and Molecular Biology0 (SwePub)301082 hsv//eng |
| 653 | a aseptic loosening; calpeptin; exosomes; fluid shear stress; GW4869; microvesicles | |
| 700 | 1 | a Johansson, L.u Linköpings universitet,Institutionen för biomedicinska och kliniska vetenskaper,Medicinska fakulteten,Region Östergötland, Klinisk patologi4 aut0 (Swepub:liu)n/a |
| 700 | 1 | a Liszka, Anetau Linköpings universitet,Avdelningen för cellbiologi,Medicinska fakulteten4 aut0 (Swepub:liu)aneli97 |
| 700 | 1 | a Bakker, A. D.u Univ Amsterdam, Netherlands; Vrije Univ Amsterdam, Netherlands4 aut |
| 700 | 1 | a Hallbeck, Martinu Linköpings universitet,Avdelningen för neurobiologi,Medicinska fakulteten,Region Östergötland, Klinisk patologi4 aut0 (Swepub:liu)marha90 |
| 700 | 1 | a Fahlgren, Annau Linköpings universitet,Avdelningen för cellbiologi,Medicinska fakulteten4 aut0 (Swepub:liu)annfa39 |
| 710 | 2 | a Linköpings universitetb Avdelningen för cellbiologi4 org |
| 773 | 0 | t The FASEB Journald : WILEYg 38:1q 38:1x 0892-6638x 1530-6860 |
| 856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-199533 |
| 856 | 4 8 | u https://doi.org/10.1096/fj.202301520R |
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