Sökning: onr:"swepub:oai:DiVA.org:liu-25224" > A Lectin Immunosens...
Fältnamn | Indikatorer | Metadata |
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000 | 02873naa a2200385 4500 | |
001 | oai:DiVA.org:liu-25224 | |
003 | SwePub | |
008 | 091007s2001 | |||||||||||000 ||eng| | |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-252242 URI |
024 | 7 | a https://doi.org/10.1006/abio.2000.49052 DOI |
040 | a (SwePub)liu | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Liljeblad, Mathiasu Linköpings universitet,Klinisk kemi,Hälsouniversitetet4 aut0 (Swepub:liu)matli01x |
245 | 1 0 | a A Lectin Immunosensor Technique for Determination of α1-Acid Glycoprotein Fucosylation |
264 | 1 | b Elsevier BV,c 2001 |
338 | a print2 rdacarrier | |
520 | a The fucosylation of α1-acid glycoprotein (AGP), an acute-phase protein, is known to change in association with inflammatory diseases. Thus, fucosylation of AGP could be a potential diagnostic or prognostic marker. The change in fucosylation has previously been investigated using crossed affinoimmunoelectrophoresis, high-pH anion-exchange chromatography, and lectin ELISA. This study describes a surface plasmon resonance-based affinity biosensor assay for quantification of the fucosylation of AGP. Diluted EDTA plasma or serum was injected directly in a BIACORE 2000 biosensor. AGP was captured on the sensor surface using immobilized antibodies and a fucose-binding lectin from Aleuria aurentia was then used for the detection of fucosylation. The feature of the biosensor makes it possible to determine both the amount of bound AGP and the amount of bound lectin. Using a calibration curve it was possible to obtain a fucosylation ratio that was independent of AGP concentration. The assay was validated against a lectin ELISA and used to follow inflammation in patients with severe burns. | |
653 | a a1-acid glycoprotein | |
653 | a Aleuria aurentia lectin | |
653 | a Fucose | |
653 | a Glycosylation | |
653 | a Surface plasmon resonance | |
653 | a MEDICINE | |
653 | a MEDICIN | |
700 | 1 | a Rydén, Ingvaru Department of Clinical Chemistry, Kalmar County Hospital, Kalmar, Sweden4 aut |
700 | 1 | a Ohlson, Stenu Division of Biochemistry/Biotechnology, Department of Chemistry and Biomedical Sciences, University of Kalmar, Kalmar, Sweden4 aut |
700 | 1 | a Lundblad, Arne,d 1937-u Linköpings universitet,Klinisk kemi,Hälsouniversitetet4 aut0 (Swepub:liu)arnlu37 |
700 | 1 | a Påhlsson, Peter,d 1962-u Linköpings universitet,Klinisk kemi,Hälsouniversitetet4 aut0 (Swepub:liu)petpa61 |
710 | 2 | a Linköpings universitetb Klinisk kemi4 org |
773 | 0 | t Analytical Biochemistryd : Elsevier BVg 288:2, s. 216-224q 288:2<216-224x 0003-2697x 1096-0309 |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-25224 |
856 | 4 8 | u https://doi.org/10.1006/abio.2000.4905 |
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