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High-Resolution Mel...
High-Resolution Melting-Curve Analysis of Ligation-Mediated Real-Time PCR for Rapid Evaluation of an Epidemiological Outbreak of Extended-Spectrum-Beta-Lactamase-Producing Escherichia coli
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- Woksepp, Hanna (författare)
- Kalmar County Hospital
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- Jernberg, Cecilia (författare)
- Swedish Institute for Communicable Disease Control, Solna
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- Tärnberg, Maria (författare)
- Linköpings universitet,Klinisk mikrobiologi,Hälsouniversitetet
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- Ryberg, Anna (författare)
- Östergötlands Läns Landsting,Linköpings universitet,Institutionen för klinisk och experimentell medicin,Hälsouniversitetet,Klinisk mikrobiologi
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- Brolund, Alma (författare)
- Karolinska Institutet
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- Nordvall, Michaela (författare)
- Östergötlands Läns Landsting,Klinisk mikrobiologi
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- Olsson-Liljequist, Barbro (författare)
- Swedish Institute for Communicable Disease Control, Solna
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- Tegmark Wisell, Karin (författare)
- Swedish Institute for Communicable Disease Control, Solna
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- Monstein, Hans-Jurg (författare)
- Östergötlands Läns Landsting,Linköpings universitet,Klinisk mikrobiologi,Hälsouniversitetet
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- Nilsson, Lennart E (författare)
- Linköpings universitet,Klinisk mikrobiologi,Hälsouniversitetet
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- Schön, Thomas (författare)
- Kalmar County Hospital
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(creator_code:org_t)
- American Society for Microbiology, 2011
- 2011
- Engelska.
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Ingår i: Journal of Clinical Microbiology. - : American Society for Microbiology. - 0095-1137 .- 1098-660X. ; 49:12, s. 4032-4039
- Relaterad länk:
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https://liu.diva-por... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.1...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- Methods for the confirmation of nosocomial outbreaks of bacterial pathogens are complex, expensive, and time-consuming. Recently, a method based on ligation-mediated PCR (LM/PCR) using a low denaturation temperature which produces specific melting-profile patterns of DNA products has been described. Our objective was to further develop this method for real-time PCR and high-resolution melting analysis (HRM) in a single-tube system optimized in order to achieve results within 1 day. Following the optimization of LM/PCR for real-time PCR and HRM (LM/HRM), the method was applied for a nosocomial outbreak of extended-spectrum-beta-lactamase (ESBL)-producing and ST131-associated Escherichia coli isolates (n = 15) and control isolates (n = 29), including four previous clusters. The results from LM/HRM were compared to results from pulsed-field gel electrophoresis (PFGE), which served as the gold standard. All isolates from the nosocomial outbreak clustered by LM/HRM, which was confirmed by gel electrophoresis of the LM/PCR products and PFGE. Control isolates that clustered by LM/PCR (n = 4) but not by PFGE were resolved by confirmatory gel electrophoresis. We conclude that LM/HRM is a rapid method for the detection of nosocomial outbreaks of bacterial infections caused by ESBL-producing E. coli strains. It allows the analysis of isolates in a single-tube system within a day, and the discriminatory power is comparable to that of PFGE.
Nyckelord
- MEDICINE
- MEDICIN
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Woksepp, Hanna
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Jernberg, Cecili ...
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Tärnberg, Maria
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Ryberg, Anna
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Brolund, Alma
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Nordvall, Michae ...
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visa fler...
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Olsson-Liljequis ...
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Tegmark Wisell, ...
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Monstein, Hans-J ...
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Nilsson, Lennart ...
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Schön, Thomas
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- Artiklar i publikationen
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Journal of Clini ...
- Av lärosätet
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Linköpings universitet
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Karolinska Institutet