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Second Messenger Fu...
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Gasser, AndreasUniversity Medical Center Hamburg-Eppendorf, Germany
(författare)
Second Messenger Function of Nicotinic Acid Adenine Dinucleotide Phosphate Revealed by an Improved Enzymatic Cycling Assay
- Artikel/kapitelEngelska2006
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American Society for Biochemistry and Molecular Biology,2006
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LIBRIS-ID:oai:DiVA.org:liu-81990
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https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-81990URI
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https://doi.org/10.1074/jbc.M601347200DOI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
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Nicotinic acid adenine dinucleotide phosphate (NAADP) is the most potent activator of Ca2+ release from intracellular stores known today. Although recent reports have suggested an important function of NAADP in human T lymphocytes, direct evidence for receptor-induced formation of NAADP is yet missing in these cells. Thus, we developed a highly sensitive and specific enzyme assay capable of quantifying low fmol amounts of NAADP. In unstimulated T cells, the NAADP concentration amounted to 4.4 +/- 1.6 nm (0.055 +/- 0.028 pmol/mg of protein). Stimulation of the cells via the T cell receptor/CD3 complex resulted in biphasic elevation kinetics of cellular NAADP levels and was characterized by a bell-shaped concentration-response curve for NAADP. In contrast, the NAADP concentration was elevated neither upon activation of the ADP-ribose/TRPM2 channel Ca2+ signaling system nor by an increase of the intracellular Ca2+ concentration upon thapsigargin stimulation. T cell receptor/CD3 complex-mediated NAADP formation was dependent on the activity of tyrosine kinases because genistein completely blocked NAADP elevation. Thus, we propose a regulated formation of NAADP upon specific stimulation of the T cell receptor/CD3 complex, suggesting a function of NAADP as a Ca2+-mobilizing second messenger during T cell activation.
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Bruhn, Sören,1976-University Medical Center Hamburg-Eppendorf, Germany(Swepub:liu)sorbr27
(författare)
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Guse, AndreasUniversity Medical Center Hamburg-Eppendorf, Germany
(författare)
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University Medical Center Hamburg-Eppendorf, Germany
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Journal of Biological Chemistry: American Society for Biochemistry and Molecular Biology281:25, s. 16906-169130021-92581083-351X
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