onr:"swepub:oai:DiVA.org:liu-86856"
Sökning: onr:"swepub:oai:DiVA.org:liu-86856" > An efficient approa...
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| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 03889naa a2200409 4500 | |
| 001 | oai:DiVA.org:liu-86856 | |
| 003 | SwePub | |
| 008 | 130107s2010 | |||||||||||000 ||eng| | |
| 024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-868562 URI |
| 024 | 7 | a https://doi.org/10.1007/s00216-010-4153-22 DOI |
| 040 | a (SwePub)liu | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a ref2 swepub-contenttype |
| 072 | 7 | a art2 swepub-publicationtype |
| 100 | 1 | a Gökay, O.u Institute of Organic Chemistry, University of Tübingen, Germany4 aut |
| 245 | 1 0 | a An efficient approach for the isolation, identification and evaluation of antimicrobial plant components on an analytical scale, demonstrated by the example of Radix imperatoriae |
| 264 | c 2010-09-09 | |
| 264 | 1 | b Springer,c 2010 |
| 338 | a electronic2 rdacarrier | |
| 520 | a Using Radix imperatoriae (the root of masterwort) as an example, we describe an efficient approach for the isolation, identification and evaluation of bioactive plant components on an analytical scale. The extraction of Radix imperatoriae with ethyl acetate was enhanced by the application of ultrasound oscillations. This rhizome extract was applied to three pathogenic bacteria ( Bacillus cereus, Escherichia coli, and Staphylococcus aureus) to determine its antimicrobial activity. Disk diffusion was utilized to determine susceptibility. The extract components were separated using a series of chromatography approaches (semi-preparative RP-HPLC, or RP-HPLC on an analytical scale), followed by testing. All fractions were analyzed by LC-UV-ESI-MS and 600 MHz microcoil H NMR spectroscopy. Among other findings, in the fraction with the highest antibacterial activity we were able to identify oxypeucedanin and oxypeucedanin hydrate. Subsequent analysis revealed that only oxypeucedanin hydrate had antibacterial activity, whereas oxypeucedanin itself was inactive at the concentrations applied. Furthermore, oxypeucedanin hydrate appears to be largely, or exclusively, a by-product of sample preparation, since it is either not synthesized by the plant as a second metabolite or is produced by it in only very small quantities. | |
| 650 | 7 | a NATURVETENSKAPx Kemix Analytisk kemi0 (SwePub)104012 hsv//swe |
| 650 | 7 | a NATURAL SCIENCESx Chemical Sciencesx Analytical Chemistry0 (SwePub)104012 hsv//eng |
| 650 | 7 | a NATURVETENSKAPx Biologix Biokemi och molekylärbiologi0 (SwePub)106022 hsv//swe |
| 650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Biochemistry and Molecular Biology0 (SwePub)106022 hsv//eng |
| 653 | a Radix imperatoriae . Peucedanum ostruthium . Antimicrobial activity . LC-ESI-MS . Microcoil NMR | |
| 700 | 1 | a Kühner, D.u Institute of Microbiology and Infectious Diseases, University of Tübingen, Germany4 aut |
| 700 | 1 | a Los, Marek Janu Interfaculty Institute for Biochemistry, University of Tübingen, Germany4 aut0 (Swepub:liu)marlo14 |
| 700 | 1 | a Götz, F.u Institute of Microbiology and Infectious Diseases, University of Tübingen, Germany4 aut |
| 700 | 1 | a Bertsche, U.u Institute of Microbiology and Infectious Diseases, University of Tübingen, Gemany4 aut |
| 700 | 1 | a Albert, K.u Institute of Organic Chemistry, University of Tübingen, Germany4 aut |
| 710 | 2 | a Institute of Organic Chemistry, University of Tübingen, Germanyb Institute of Microbiology and Infectious Diseases, University of Tübingen, Germany4 org |
| 773 | 0 | t Analytical and Bioanalytical Chemistryd : Springerg 398:5, s. 2039-2047q 398:5<2039-2047x 1618-2642x 1618-2650 |
| 856 | 4 | u https://liu.diva-portal.org/smash/get/diva2:582928/FULLTEXT01.pdfx primaryx Raw objecty fulltext:preprint |
| 856 | 4 | u http://liu.diva-portal.org/smash/get/diva2:582928/FULLTEXT01 |
| 856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-86856 |
| 856 | 4 8 | u https://doi.org/10.1007/s00216-010-4153-2 |
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