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| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 04109naa a2200469 4500 | |
| 001 | oai:DiVA.org:ltu-78018 | |
| 003 | SwePub | |
| 008 | 200310s2019 | |||||||||||000 ||eng| | |
| 024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:ltu:diva-780182 URI |
| 024 | 7 | a https://doi.org/10.1186/s13068-019-1628-z2 DOI |
| 040 | a (SwePub)ltu | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a ref2 swepub-contenttype |
| 072 | 7 | a art2 swepub-publicationtype |
| 100 | 1 | a Karnaouri, Anthi C.u Luleå tekniska universitet,Kemiteknik4 aut0 (Swepub:ltu)antkar |
| 245 | 1 0 | a Valorization of waste forest biomass toward the production of cello-oligosaccharides with potential prebiotic activity by utilizing customized enzyme cocktails |
| 264 | c 2019-12-10 | |
| 264 | 1 | b BioMed Central (BMC),c 2019 |
| 338 | a print2 rdacarrier | |
| 500 | a Validerad;2020;Nivå 2;2020-03-10 (johcin) | |
| 520 | a BackgroundProduction of value-added materials from lignocellulosic biomass residues is an emerging sector that has attracted much attention as it offers numerous benefits from an environmental and economical point of view. Non-digestible oligosaccharides represent a group of carbohydrates that are resistant to gastrointestinal digestion, and therefore, they are considered as potential prebiotic candidates. Such oligosaccharides can derive from the biomass cellulose fraction through a controlled enzymatic hydrolysis that eliminates the yield of monomers.ResultsIn the present study, hydrolysis of organosolv-pretreated forest residues (birch and spruce) was tested in the presence of four cellulases (EG5, CBH7, CBH6, EG7) and one accessory enzyme (LPMO). The optimal enzyme combinations were comprised of 20% EG5, 43% CBH7, 22% TtLPMO, 10% PaCbh6a and 5% EG7 in the case of birch and 35% EG5, 45% CBH7, 10% TtLPMO, 10% PaCbh6a and 5% EG7 in the case of spruce, leading to 22.3% and 19.1 wt% cellulose conversion into cellobiose, respectively. Enzymatic hydrolysis was applied on scale-up reactions, and the produced oligosaccharides (consisted of > 90% cellobiose) were recovered and separated from glucose through nanofiltration at optimized temperature (50 °C) and pressure (10 bar) conditions, yielding a final product with cellobiose-to-glucose ratio of 21.1 (birch) and 20.2 (spruce). Cellobiose-rich hydrolysates were tested as fermentative substrates for different lactic acid bacteria. It was shown that they can efficiently stimulate the growth of two Lactobacilli strains.ConclusionsControlled enzymatic hydrolysis with processive cellulases, combined with product recovery and purification, as well as enzyme recycling can potentially support the sustainable production of food-grade oligosaccharides from forest biomass. | |
| 650 | 7 | a TEKNIK OCH TEKNOLOGIERx Industriell bioteknikx Bioprocessteknik0 (SwePub)209012 hsv//swe |
| 650 | 7 | a ENGINEERING AND TECHNOLOGYx Industrial Biotechnologyx Bioprocess Technology0 (SwePub)209012 hsv//eng |
| 653 | a Non-digestible oligosaccharides | |
| 653 | a Cellobiose | |
| 653 | a Processive endoglucanases | |
| 653 | a Prebiotics | |
| 653 | a Enzyme hydrolysis | |
| 653 | a Nanofiltration | |
| 653 | a Biokemisk processteknik | |
| 653 | a Biochemical Process Engineering | |
| 700 | 1 | a Matsakas, Leonidasu Luleå tekniska universitet,Kemiteknik4 aut0 (Swepub:ltu)leomat |
| 700 | 1 | a Krikigianni, Eleniu Luleå tekniska universitet,Kemiteknik4 aut0 (Swepub:ltu)elekri |
| 700 | 1 | a Rova, Ulrikau Luleå tekniska universitet,Kemiteknik4 aut0 (Swepub:ltu)ulrok |
| 700 | 1 | a Christakopoulos, Paulu Luleå tekniska universitet,Kemiteknik4 aut0 (Swepub:ltu)pauchr |
| 710 | 2 | a Luleå tekniska universitetb Kemiteknik4 org |
| 773 | 0 | t Biotechnology for Biofuelsd : BioMed Central (BMC)g 12:1q 12:1x 1754-6834 |
| 856 | 4 | u https://doi.org/10.1186/s13068-019-1628-zy Fulltext |
| 856 | 4 | u https://biotechnologyforbiofuels.biomedcentral.com/track/pdf/10.1186/s13068-019-1628-z |
| 856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:ltu:diva-78018 |
| 856 | 4 8 | u https://doi.org/10.1186/s13068-019-1628-z |
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