onr:"swepub:oai:DiVA.org:sh-31883"
Sökning: onr:"swepub:oai:DiVA.org:sh-31883" > An alternate patter...
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| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 03854naa a2200385 4500 | |
| 001 | oai:DiVA.org:sh-31883 | |
| 003 | SwePub | |
| 008 | 170127s2005 | |||||||||||000 ||eng| | |
| 009 | oai:prod.swepub.kib.ki.se:1958224 | |
| 024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:sh:diva-318832 URI |
| 024 | 7 | a https://doi.org/10.1021/bi047480h2 DOI |
| 024 | 7 | a http://kipublications.ki.se/Default.aspx?queryparsed=id:19582242 URI |
| 040 | a (SwePub)shd (SwePub)ki | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a ref2 swepub-contenttype |
| 072 | 7 | a art2 swepub-publicationtype |
| 100 | 1 | a Yildirim, Håkan H.u Södertörns högskola,Institutionen för livsvetenskaper,Karolinska Institutet4 aut0 (Swepub:sh)SHHNYM |
| 245 | 1 0 | a An alternate pattern for globoside oligosaccharide expression in Haemophilus influenzae lipopolysaccharide :b Structural diversity in nontypeable strain 1124 |
| 264 | c 2005-03-08 | |
| 264 | 1 | b American Chemical Society (ACS),c 2005 |
| 338 | a print2 rdacarrier | |
| 520 | a Common structural motifs of Haemophilus influenzae lipopolysaccharide (LPS) are globotetraose [beta-D-GalpNAc-(1 -> 3)-alpha-D-Galp-(1 -> 4)-beta-D-Galp-(1 -> 4)-beta-D-Glcp] and its truncated versions globoside [alpha-D-Galp-(1 -> 4)-beta-D-Galp-(1 -> 4)-beta-D-Glcp] and lactose [beta-D-Galp-(1 -> 4)-beta-D-Glcp] linked to the tenninal heptose (HepIII) of the triheptosyl inner-core moiety L-alpha-D-Hepp-(1 -> 2)-[PEA -> 6]-L-alpha-D-Hepp-(1 -> 3)L-alpha-D-Hepp-(1 -> 5)-[PPEA -> 4]-alpha-Kdo-(2 -> 6)-lipid A. We report here structural studies of LPS from nontypeable H. influenzae strain 1124 expressing these motifs linked to both the proximal heptose (HepI) and HepIII at the same time. This novel finding was obtained by structural studies of LPS using NMR techniques and electrospray ionization mass spectrometry (ESI-MS) on O-deacylated LPS and core oligosaccharide material (OS) as well as ESI-MSn on permethylated dephosphorylated OS. The use of defined mutants allowed us to confirm structures unambiguously and understand better the biosynthesis of each of the globotetraose units. We found that lgtC is involved in the expression of beta-D-Galp-(1 -> 4)-beta-D-Galp in both extensions, whereas lic2A directs only the expression Of beta-D-Ga1p-(1 -> 4)-beta-D-Glcp when linked to HepIII. The LPS of NTHi strain 1124 contained sialylated glycoforms that were identified by CE-ESI-MS/MS. A common sialylated structure in H. influenzae LPS is sialyllactose linked to HepIII. This structure exists in strain 1124. However, results for the lpsA mutant indicate that sialyllactose extends from HepI as well, a molecular environment for sialyllactose in H. influenzae that has not been reported previously. In addition, the LPS was found to carry phosphoryleholine, O-linked glycine, and a third PEA group which was linked to O3 of HepIII. | |
| 650 | 7 | a NATURVETENSKAPx Biologi0 (SwePub)1062 hsv//swe |
| 650 | 7 | a NATURAL SCIENCESx Biological Sciences0 (SwePub)1062 hsv//eng |
| 700 | 1 | a Li, J Ju National Research Council of Canada, Ottawa, Canada4 aut |
| 700 | 1 | a Richards, J Cu National Research Council of Canada, Ottawa, Canada4 aut |
| 700 | 1 | a Hood, D Wu John Radcliffe Hospital, Oxford, UK4 aut |
| 700 | 1 | a Moxon, E Ru John Radcliffe Hospital, Oxford, UK4 aut |
| 700 | 1 | a Schweda, Elke K. H.u Södertörns högskola,Institutionen för livsvetenskaper,Karolinska Institutet4 aut0 (Swepub:sh)SHEESA |
| 710 | 2 | a Södertörns högskolab Institutionen för livsvetenskaper4 org |
| 773 | 0 | t Biochemistryd : American Chemical Society (ACS)g 44:13, s. 5207-5224q 44:13<5207-5224x 0006-2960x 1520-4995 |
| 856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:sh:diva-31883 |
| 856 | 4 8 | u https://doi.org/10.1021/bi047480h |
| 856 | 4 8 | u http://kipublications.ki.se/Default.aspx?queryparsed=id:1958224 |
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