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Chromosome Level As...
Chromosome Level Assembly of the Comma Butterfly (Polygonia c-album)
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- Celorio-Mancera, Maria de la Paz (författare)
- Stockholms universitet,Zoologiska institutionen
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Rastas, Pasi (författare)
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- Steward, Rachel A. (författare)
- Stockholms universitet,Zoologiska institutionen
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- Nylin, Sören (författare)
- Stockholms universitet,Zoologiska institutionen
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- Wheat, Christopher W. (författare)
- Stockholms universitet,Zoologiska institutionen
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(creator_code:org_t)
- 2021-03-22
- 2021
- Engelska.
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Ingår i: Genome Biology and Evolution. - : Oxford University Press (OUP). - 1759-6653. ; 13:5
- Relaterad länk:
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https://doi.org/10.1...
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https://academic.oup...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- The comma butterfly (Polygonia c-album, Nymphalidae, Lepidoptera) is a model insect species, most notably in the study of phenotypic plasticity and plant-insect coevolutionary interactions. In order to facilitate the integration of genomic tools with a diverse body of ecological and evolutionary research, we assembled the genome of a Swedish comma using 10X sequencing, scaffolding with matepair data, genome polishing, and assignment to linkage groups using a high-density linkage map. The resulting genome is 373 Mb in size, with a scaffold N50 of 11.7 Mb and contig N50 of 11,2Mb. The genome contained 90.1% of single-copy Lepidopteran orthologs in a BUSCO analysis of 5,286 genes. A total of 21,004 gene-models were annotated on the genome using RNA-Seq data from larval and adult tissue in combination with proteins from the Arthropoda database, resulting in a high-quality annotation for which functional annotations were generated. We further documented the quality of the chromosomal assembly via synteny assessment with Melitaea cinxia. The resulting annotated, chromosome-level genome will provide an important resource for investigating coevolutionary dynamics and comparative analyses in Lepidoptera.
Ämnesord
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Nyckelord
- linkage map
- butterfly genome
- quantitative annotation assessment
- comparative genomics
- Polygonia c-album
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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