SwePub
Sök i LIBRIS databas

  Extended search

onr:"swepub:oai:DiVA.org:su-199263"
 

Search: onr:"swepub:oai:DiVA.org:su-199263" > PplD is a de-N-acet...

  • 1 of 1
  • Previous record
  • Next record
  •    To hitlist
LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00003029nam a2200469 4500
001oai:DiVA.org:su-199263
003SwePub
008211130s2021 | |||||||||||000 ||eng|
0247 a https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-1992632 URI
0247 a https://doi.org/10.1101/2021.09.23.4615902 DOI
040 a (SwePub)su
041 a engb eng
042 9 SwePub
072 7a vet2 swepub-contenttype
072 7a ovr2 swepub-publicationtype
1001 a Rush, Jeffrey S.4 aut
2451 0a PplD is a de-N-acetylase of the cell wall linkage unit of streptococcal rhamnopolysaccharides
264 1b Cold Spring Harbor Laboratory,c 2021
338 a print2 rdacarrier
520 a The cell wall of the human bacterial pathogen Group A Streptococcus (GAS) consists of peptidoglycan decorated with the Lancefield group A carbohydrate (GAC). GAC is a promising target for the development of GAS vaccines. In this study, employing chemical, compositional, and NMR methods, we show that GAC is attached to peptidoglycan via glucosamine 1-phosphate. This structural feature makes the GAC-peptidoglycan linkage highly sensitive to cleavage by nitrous acid and resistant to mild acid conditions. Using this characteristic of the GAS cell wall, we identify PplD as a protein required for deacetylation of linkage N-acetylglucosamine (GlcNAc). X-ray structural analysis indicates that PplD performs catalysis via a modified acid/base mechanism. Genetic surveys in silico together with functional analysis indicate that PplD homologs deacetylate the polysaccharide linkage in many streptococcal species. We further demonstrate that introduction of positive charges to the cell wall by GlcNAc deacetylation protects GAS against host cationic antimicrobial proteins.
650 7a NATURVETENSKAPx Biologix Biokemi och molekylärbiologi0 (SwePub)106022 hsv//swe
650 7a NATURAL SCIENCESx Biological Sciencesx Biochemistry and Molecular Biology0 (SwePub)106022 hsv//eng
653 a biokemi
653 a Biochemistry
7001 a Parajuli, Prakash4 aut
7001 a Ruda, Alessandro,d 1990-u Stockholms universitet,Institutionen för organisk kemi4 aut0 (Swepub:su)alru3031
7001 a Li, Jian4 aut
7001 a Pohane, Amol A.4 aut
7001 a Zamakhaeva, Svetlana4 aut
7001 a Rahman, Mohammad M.4 aut
7001 a Chang, Jennifer C.4 aut
7001 a Gogos, Artemis4 aut
7001 a Kenner, Cameron W.4 aut
7001 a Lambeau, Gérard4 aut
7001 a Federle, Michael J.4 aut
7001 a Korotkov, Konstantin V.4 aut
7001 a Widmalm, Göranu Stockholms universitet,Institutionen för organisk kemi4 aut0 (Swepub:su)gw
7001 a Korotkova, Natalia4 aut
7102 a Stockholms universitetb Institutionen för organisk kemi4 org
8564 u https://uknowledge.uky.edu/cgi/viewcontent.cgi?article=1195&context=biochem_facpub
8564 8u https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-199263
8564 8u https://doi.org/10.1101/2021.09.23.461590

To the university's database

  • 1 of 1
  • Previous record
  • Next record
  •    To hitlist

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Close

Copy and save the link in order to return to this view