Staphylococcus aureus-derived factors promote human Th9 cell polarization and enhance a transcriptional program associated with allergic inflammation

Badolati, Isabella (author): Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut

van Der Heiden, Marieke (author): Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut,University of Groningen, Netherlands

Zuurveld, Marit (author): Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut

Szilágyi, Szilvia (author): Karolinska Institutet,Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut

Björkander, Sophia, 1987- (author): Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut,Karolinska Institutet, Sweden

Sverremark-Ekström, Eva, 1968- (author): Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut

(creator_code:org_t)

2023-01-17
2023
English.
In: European Journal of Immunology. - : Wiley. - 0014-2980 .- 1521-4141. ; 53:3

Related links:: https://doi.org/10.1...; show more...; https://urn.kb.se/re...; https://doi.org/10.1...; http://kipublication...; show less...

Abstract Subject headings

T helper (Th) 9 cells, characterized by robust secretion of IL-9, have been increasingly associated with allergic diseases. However, whether and how Th9 cells are modulated by environmental stimuli remains poorly understood. In this study, we show that in vitro exposure of human PBMCs or isolated CD4 T-cells to Staphylococcus (S.) aureus-derived factors, including its toxins, potently enhances Th9 cell frequency and IL-9 secretion. Furthermore, as revealed by RNA sequencing analysis, S. aureus increases the expression of Th9-promoting factors at the transcriptional level, such as FOXO1, miR-155, and TNFRSF4. The addition of retinoic acid (RA) dampens the Th9 responses promoted by S. aureus and substantially changes the transcriptional program induced by this bacterium, while also altering the expression of genes associated with allergic inflammation. Together, our results demonstrate a strong influence of microbial and dietary factors on Th9 cell polarization, which may be important in the context of allergy development and treatment.

By the author/editor: Badolati, Isabel ...; van Der Heiden, ...; Brodin, David; Zuurveld, Marit; Szilágyi, Szilvi ...; Björkander, Soph ...; show more...; Sverremark-Ekstr ...; show less...

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Immunology in th ...

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Clinical Medicin ...; and Respiratory Medi ...

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