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| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 02672naa a2200385 4500 | |
| 001 | oai:DiVA.org:umu-156612 | |
| 003 | SwePub | |
| 008 | 190220s2019 | |||||||||||000 ||eng| | |
| 024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-1566122 URI |
| 024 | 7 | a https://doi.org/10.1016/j.jchromb.2018.11.0072 DOI |
| 040 | a (SwePub)umu | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a ref2 swepub-contenttype |
| 072 | 7 | a art2 swepub-publicationtype |
| 100 | 1 | a Claeson, Anna-Sara,d 1974-u Umeå universitet,Institutionen för psykologi4 aut0 (Swepub:umu)ancl0002 |
| 245 | 1 0 | a A standardized protocol for comparable analysis of GSH/GSSG by UHPLC-ESI-MSMS for human plasma |
| 264 | 1 | b Elsevier,c 2019 |
| 338 | a print2 rdacarrier | |
| 520 | a Variability in the levels of GSH and GSSG in plasma is suggested to derive from inadequate pre-processing methods. The aim of this study was to develop a protocol for comparable and reliable measurements of GSH/GSSG. Venous blood from 8 healthy individuals were collected and divided into 7 different pre-processing procedures. For three of the samples an extraction mixture was added after 0 (baseline), 4 and 8 min and for three of the samples the extraction mixture was added at different times during defrost. A worst case scenario where a sample was left in a cool box during 6 h was also included. The samples were analyzed with UHPLC-ESIMSMS. A large difference in the levels of GSH and GSSG were identified and it was clearly associated with the sample handling procedures. A sample left untreated for 4 min will have significantly reduced amount of GSH. Stability tests showed that the level of GSH was reduced after 3 months in -80 degrees C. | |
| 650 | 7 | a NATURVETENSKAPx Kemix Analytisk kemi0 (SwePub)104012 hsv//swe |
| 650 | 7 | a NATURAL SCIENCESx Chemical Sciencesx Analytical Chemistry0 (SwePub)104012 hsv//eng |
| 653 | a GIutathione | |
| 653 | a GSH | |
| 653 | a GSSG | |
| 653 | a Plasma | |
| 653 | a Sample pre-processing | |
| 653 | a UHPLC-ESI-MS | |
| 700 | 1 | a Gouveia-Figueira, Sandra C.u Swedish Metabolomics Centre (SMC), Umeå, Sweden4 aut0 (Swepub:umu)sago0010 |
| 700 | 1 | a Stenlund, Hansu Swedish Metabolomics Centre (SMC), Umeå, Sweden4 aut0 (Swepub:umu)hasstd97 |
| 700 | 1 | a Johansson, Annika I.u Swedish Metabolomics Centre (SMC), Umeå, Sweden4 aut |
| 710 | 2 | a Umeå universitetb Institutionen för psykologi4 org |
| 773 | 0 | t Journal of chromatography. Bd : Elsevierg 1104, s. 67-72q 1104<67-72x 1570-0232x 1873-376X |
| 856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-156612 |
| 856 | 4 8 | u https://doi.org/10.1016/j.jchromb.2018.11.007 |
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