onr:"swepub:oai:DiVA.org:umu-165889"
Search: onr:"swepub:oai:DiVA.org:umu-165889" > A NIMA-related prot...
- 1 of 1
- Previous record
- Next record
- To hitlist
A NIMA-related protein kinase is essential for completion of the sexual cycle of malaria parasites
- Reininger, Luc (author)
- Billker, Oliver (author)
- Tewari, Rita (author)
- show more...
- Mukhopadhyay, Arunima (author)
- Fennell, Clare (author)
- Dorin-Semblat, Dominique (author)
- Doerig, Caroline (author)
- Goldring, Dean (author)
- Harmse, Leonie (author)
- Ranford-Cartwright, Lisa (author)
- Packer, Jeremy (author)
- Doerig, Christian (author)
- show less...
- 2005
- 2005
- English.
- In: Journal of Biological Chemistry. - 0021-9258 .- 1083-351X. ; 280:36, s. 31957-31964
- Journal article (peer-reviewed)
Abstract
Subject headings
Close
- The molecular mechanisms regulating the sexual development of malaria parasites from gametocytes to oocysts in their mosquito vector are still largely unexplored. In other eukaryotes, NIMA-related kinases (Neks) regulate cell cycle progression and have been implicated in the regulation of meiosis. Here, we demonstrate that Nek-4, a new Plasmodium member of the Nek family, is essential for completion of the sexual cycle of the parasite. Recombinant Plasmodium falciparum Nek-4 possesses protein kinase activity and displays substrate preferences similar to those of other Neks. Nek-4 is highly expressed in gametocytes, yet disruption of the nek-4 gene in the rodent malaria parasite P. berghei has no effect on gamete formation and subsequent fertilization. However, further differentiation of zygotes into ookinetes is abolished. Measurements of nuclear DNA content indicate that zygotes lacking Nek-4 fail to undergo the genome replication to the tetraploid level that precedes meiosis. Cell cycle progression in the zygote is identified as a likely precondition for its morphological transition to the ookinete and for the successful establishment of a malaria infection in the mosquito.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- Amino Acid Sequence
- Animals
- Catalytic Domain
- Cell Cycle/*physiology
- DNA Replication/physiology
- Evolution
- Molecular
- Humans
- Life Cycle Stages/*physiology
- Meiosis/physiology
- Molecular Sequence Data
- NIMA-Related Kinases
- Phylogeny
- Plasmodium falciparum/*enzymology/genetics/*growth & development
- Protein Kinases/genetics/*physiology
- Protein-Serine-Threonine Kinases
- Protozoan Proteins/genetics/*physiology
- RNA
- Messenger/metabolism
- Sequence Alignment
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
- Journal of Biological Chemistry (Search for host publication in LIBRIS)
To the university's database
- 1 of 1
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Reininger, Luc
- Billker, Oliver
- Tewari, Rita
- Mukhopadhyay, Ar ...
- Fennell, Clare
- Dorin-Semblat, D ...
- show more...
- Doerig, Caroline
- Goldring, Dean
- Harmse, Leonie
- Ranford-Cartwrig ...
- Packer, Jeremy
- Doerig, Christia ...
- show less...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
- MEDICAL AND HEAL ...
- and Basic Medicine
- and Cell and Molecul ...
- Articles in the publication
- Journal of Biolo ...
- By the university
- Umeå University
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
