onr:"swepub:oai:DiVA.org:umu-40751"
Sökning: onr:"swepub:oai:DiVA.org:umu-40751" > Tubulin-α-6-chain i...
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| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 03207naa a2200445 4500 | |
| 001 | oai:DiVA.org:umu-40751 | |
| 003 | SwePub | |
| 008 | 110309s2010 | |||||||||||000 ||eng| | |
| 024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-407512 URI |
| 024 | 7 | a https://doi.org/10.3892/etm_000000652 DOI |
| 040 | a (SwePub)umu | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a ref2 swepub-contenttype |
| 072 | 7 | a art2 swepub-publicationtype |
| 100 | 1 | a Rentoft, Matildau Umeå universitet,Patologi4 aut0 (Swepub:umu)maaret01 |
| 245 | 1 0 | a Tubulin-α-6-chain is a stably expressed reference gene in archival samples of normal oral tissue and squamous cell carcinoma of the head and neck |
| 264 | c 2010-05-01 | |
| 264 | 1 | b Spandidos Publications,c 2010 |
| 338 | a print2 rdacarrier | |
| 520 | a One of the most critical factors in gene expression studies using quantitative real-time PCR is the choice of reference gene. Many of the commonly used reference genes have been shown to vary during a number of biological processes as well as between tissues. It is therefore important to always verify the stability of the gene of choice for all new tissues and experimental conditions. Here, we used two publicly available computer software packages (GeNorm and NormFinder) to investigate the stability of eight potential reference genes in formalin-fixed paraffin-embedded (FFPE) samples from normal oral tissue of different origin as well as from oral squamous cell carcinomas. Both programs found the tubulin α-6 chain (TUBA6) and ribosomal protein S13 (RPS13) to have the most stable expression between malignant and non-malignant tissue. NormFinder also found TUBA6 to be the most stable gene when samples were grouped according to tissue origin. FFPE samples constitute a large research resource, which considerably increases the number of samples available for analysis, leading to more reliable conclusions. Verification of a proper reference gene in oral FFPE tissue is therefore of great importance for future studies. | |
| 650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Cancer och onkologi0 (SwePub)302032 hsv//swe |
| 650 | 7 | a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Cancer and Oncology0 (SwePub)302032 hsv//eng |
| 653 | a oral squamous cell carcinoma | |
| 653 | a reference gene | |
| 653 | a tubulin a-6 chain | |
| 653 | a quantitive real-time PCR | |
| 653 | a MEDICINE | |
| 653 | a MEDICIN | |
| 653 | a odontologi | |
| 653 | a Odontology | |
| 700 | 1 | a Hultin, Sarau Umeå universitet,Patologi4 aut0 (Swepub:umu)sahu0002 |
| 700 | 1 | a Coates, Philip J4 aut |
| 700 | 1 | a Laurell, Göranu Umeå universitet,Öron- näs- och halssjukdomar4 aut0 (Swepub:umu)gala0001 |
| 700 | 1 | a Nylander, Karinu Umeå universitet,Patologi4 aut0 (Swepub:umu)kaanyr77 |
| 710 | 2 | a Umeå universitetb Patologi4 org |
| 773 | 0 | t Experimental and Therapeutic Medicined : Spandidos Publicationsg 1:3, s. 419-423q 1:3<419-423x 1792-0981x 1792-1015 |
| 856 | 4 | u https://www.spandidos-publications.com/etm/1/3/419/download |
| 856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-40751 |
| 856 | 4 8 | u https://doi.org/10.3892/etm_00000065 |
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