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An antibody with hi...
An antibody with high reactivity for disease-associated α-synuclein reveals extensive brain pathology
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Kovacs, Gabor G. (författare)
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Wagner, Uta (författare)
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Dumont, Benoit (författare)
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Pikkarainen, Maria (författare)
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Osman, Awad A. (författare)
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Streichenberger, Nathalie (författare)
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Leisser, Irene (författare)
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Verchère, Jérémy (författare)
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Baron, Thierry (författare)
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- Alafuzoff, Irina (författare)
- Uppsala universitet,Molekylär och morfologisk patologi
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Budka, Herbert (författare)
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Perret-Liaudet, Armand (författare)
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Lachmann, Ingolf (författare)
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(creator_code:org_t)
- 2012-02-28
- 2012
- Engelska.
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Ingår i: Acta Neuropathologica. - : Springer Science and Business Media LLC. - 0001-6322 .- 1432-0533. ; 124:1, s. 37-50
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- α-Synuclein is the major protein associated with Lewy body dementia, Parkinson's disease and multiple system atrophy. Since α-synuclein is present in the brain in physiological conditions as a presynaptic protein, it is crucial to characterize disease-associated modifications to develop an in vivo biomarker. With the aim to develop antibodies showing high specificity and sensitivity for disease-associated α-synuclein, synthetic peptides containing different amino acid sequences were used for immunization of mice. After generation of α-synuclein aggregates, ELISA and immunoblotting were used to test the specificity of antibodies. Tissue microarray sections originating from different human α-synucleinopathies were used to compare immunostaining with other, commercially available antibodies. Immunization of mice with the peptide TKEGVVHGVATVAE (amino acid 44-57 of α-synuclein) resulted in the generation of a monoclonal antibody (5G4), which was able to bind aggregated α-synuclein preparation in sandwich ELISA or coated on magnetic beads. 5G4 proved to be superior to other antibodies in comparative immunohistochemical studies by revealing more widespread and distinct α-synuclein pathology. Immunoblotting of human brain tissue revealed an additional band seen in dementia with Lewy bodies, whereas the band representing monomeric α-synuclein was very weak or lacking. In summary, the 5G4 antibody is most promising for re-evaluation of archival material and may offer new perspective for the development of in vivo diagnostic assays for detecting disease-associated α-synuclein in body fluids.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Klinisk laboratoriemedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Clinical Laboratory Medicine (hsv//eng)
Nyckelord
- Pathology
- Patologi
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Kovacs, Gabor G.
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Wagner, Uta
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Dumont, Benoit
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Pikkarainen, Mar ...
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Osman, Awad A.
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Streichenberger, ...
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visa fler...
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Leisser, Irene
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Verchère, Jérémy
-
Baron, Thierry
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Alafuzoff, Irina
-
Budka, Herbert
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Perret-Liaudet, ...
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Lachmann, Ingolf
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visa färre...
- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Klinisk medicin
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och Klinisk laborato ...
- Artiklar i publikationen
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Acta Neuropathol ...
- Av lärosätet
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Uppsala universitet