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Immune response and...
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Camussone, C. M.
(författare)
Immune response and functional role of antibodies raised in heifers against a Staphylococcus aureus CP5 lysate and recombinant antigens vaccine formulated with Iscom Matrix adjuvant
- Artikel/kapitelEngelska2014
Förlag, utgivningsår, omfång ...
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Elsevier BV,2014
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LIBRIS-ID:oai:DiVA.org:uu-244517
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-244517URI
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https://doi.org/10.1016/j.vetimm.2014.10.004DOI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:art swepub-publicationtype
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Staphylococcus aureus is the most frequently isolated pathogen from bovine intramammary infections worldwide. Commercially available vaccines for mastitis control are composed either of S. aureus lysates or inactivated whole-cells formulated with traditional adjuvants. We recently showed the ability of a S. aureus CP5 lysate vaccine adjuvanted with Iscom Matrix to generate a longer lasting specific antibody response in blood and milk with improved opsonic capacity compared with a S. aureus CP5 whole-cell formulation. The aim of the present study was to obtain an experimental immunogen composed of lysed cells of a CP5 S. aureus strain supplemented with recombinant clumping factor A fibronectin binding protein A and beta-toxin formulated with Iscom Matrix characterize the immune response generated when immunizing pregnant heifers and assess the functional role of antibodies raised against this immunogen in experimental models. Both a lysate vaccine and a lysate + recombinant antigens vaccine elicited antibodies that promoted neutrophil phagocytosis and inhibited internalization into mammary epithelial cells in vitro. Incorporation of defined antigenic molecules to the lysate formulation elicited a strong specific humoral immune response against both lysate and recombinant antigens and was associated with higher expression of regulatory and pro-inflammatory cytokines. In addition antibodies were efficient for blocking S. aureus binding to bovine fibrinogen and fibronectin and neutralizing beta-toxin effect in vitro placing these antigens as candidates to be included in a formulation directed to prevent staphylococcal bovine mastitis. (C) 2014 Elsevier B.V. All rights reserved.
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Biuppslag (personer, institutioner, konferenser, titlar ...)
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Pujato, N.
(författare)
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Renna, M. S.
(författare)
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Veaute, C. M.
(författare)
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Morein, BrorUppsala universitet,Klinisk virologi
(författare)
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Marcipar, I. S.
(författare)
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Calvinho, L. F.
(författare)
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Uppsala universitetKlinisk virologi
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Veterinary Immunology and Immunopathology: Elsevier BV162:3-4, s. 96-1070165-24271873-2534
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