Saline catalyse oxime reaction at physiological pH: overcoming a major limitation of bioorthogonal reaction

Saline catalyse oxime reaction at physiological pH : overcoming a major limitation of bioorthogonal reaction

We have discovered a simple and versatile reaction condition for oxime mediated bioconjugation reaction that could be adapted for both aldehyde and keto substrates. We found that saline accelerated the oxime kinetics in a concentration dependent manner under physiological conditions. The reaction mechanism is validated by computational studies, and the versatility of the reaction is demonstrated by cell-surface labeling experiments. Saline offers an efficient and non-toxic catalytic option for performing the bioorthogonal-coupling reaction of biomolecules at the physiological pH. This saline mediated bioconjugation reaction represents the most bio-friendly, mild and versatile approach for conjugating sensitive biomolecules and does not require any extensive purification step.

Nawale, Ganesh N.Uppsala universitet,Polymerkemi(Swepub:uu)ganna194 (författare)
Kadekar, SandeepUppsala universitet,Polymerkemi(Swepub:uu)sanka861 (författare)
Oommen, Oommen P.Tampere University of Technology, and BioMediTech Institute, Finland (författare)
Jena, Naresh K.Uppsala universitet,Materialteori(Swepub:uu)narje540 (författare)
Chakraborty, SudipUppsala universitet,Materialteori(Swepub:uu)sudch599 (författare)
Hilborn, Jöns,1956-Uppsala universitet,Polymerkemi(Swepub:uu)johil255 (författare)
Varghese, Oommen P.,1977-Uppsala universitet,Polymerkemi(Swepub:uu)oopva021 (författare)
Uppsala universitetPolymerkemi (creator_code:org_t)

Av författaren/redakt...: Wang, Shujiang; Nawale, Ganesh N ...; Kadekar, Sandeep; Oommen, Oommen P ...; Jena, Naresh K.; Chakraborty, Sud ...; visa fler...; Hilborn, Jöns, 1 ...; Varghese, Oommen ...; visa färre...

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