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Sökning: onr:"swepub:oai:DiVA.org:uu-376902" > Immunoglobulin Gene...

LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00005422naa a2200577 4500
001oai:DiVA.org:uu-376902
003SwePub
008190212s2018 | |||||||||||000 ||eng|
009oai:prod.swepub.kib.ki.se:140119258
0247 a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-3769022 URI
0247 a https://doi.org/10.3791/577872 DOI
0247 a http://kipublications.ki.se/Default.aspx?queryparsed=id:1401192582 URI
040 a (SwePub)uud (SwePub)ki
041 a engb eng
042 9 SwePub
072 7a ref2 swepub-contenttype
072 7a art2 swepub-publicationtype
1001 a Agathangelidis, Andreasu Karolinska Institutet,Ctr Res & Technol Hellas, Inst Appl Biosci, Thessaloniki, Greece4 aut
2451 0a Immunoglobulin Gene Sequence Analysis In Chronic Lymphocytic Leukemia :b From Patient Material To Sequence Interpretation
264 c 2018-11-26
264 1b JOURNAL OF VISUALIZED EXPERIMENTS,c 2018
338 a electronic2 rdacarrier
500 a De två första författarna delar förstaförfattarskapet.
520 a During B cell maturation, the complex process of immunoglobulin (IG) gene V(D)J recombination coupled with somatic hypermutation (SHM) gives rise to a unique DNA sequence within each individual B cell. Since B cell malignancies result from the clonal expansion of a single cell, IG genes represent a unique molecular signature common to all the malignant cells within an individual patient; thus, IG gene rearrangements can be used as clonal markers. In addition to serving as an important clonal identifier, the IG gene sequence can act as a 'molecular timeline' since it is associated with specific developmental stages and hence reflects the history of the B cell involved in the neoplastic transformation. Moreover, for certain malignancies, in particular chronic lymphocytic leukemia (CLL), the IG gene sequence holds prognostic and potentially predictive capabilities. That said, extrapolating meaningful conclusions from IG gene sequence analysis would be impossible if robust methods and tools were not available to aid in their analysis. This article, drawing on the vast experience of the European Research Initiative on CLL (ERIC), details the technical aspects and essential requirements necessary to ensure reliable and reproducible IG gene sequence analysis in CLL, a test that is now recommended for all CLL patients prior to treatment. More specifically, the various analytical stages are described ranging from the identification of the clonotypic IG gene rearrangement and the determination of the nucleotide sequence to the accurate clinical interpretation of the IG gene sequence data.
650 7a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Medicinsk genetik0 (SwePub)301072 hsv//swe
650 7a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Medical Genetics0 (SwePub)301072 hsv//eng
653 a Cancer Research
653 a Issue 141
653 a Chronic lymphocytic leukemia (CLL)
653 a somatic hypermutation (SHM)
653 a immunoglobulin
653 a immunoglobulin heavy variable (IGHV)
653 a complementarity determining region 3 (CDR3)
653 a International ImMunoGene Tics information system (IMGT)
7001 a Sutton, Lesley Annu Uppsala universitet,Science for Life Laboratory, SciLifeLab,Institutionen för immunologi, genetik och patologi,Karolinska Inst, Dept Mol Med & Surg, Stockholm, Sweden4 aut0 (Swepub:uu)lessu455
7001 a Hadzidimitriou, Anastasiau Ctr Res & Technol Hellas, Inst Appl Biosci, Thessaloniki, Greece4 aut
7001 a Tresoldi, Cristinau IRCCS San Raffaele Sci Inst, Div Immunol Transplantat & Infect, Milan, Italy4 aut
7001 a Langerak, Anton W.u Erasmus Univ, Med Ctr, Lab Med Immunol, Dept Immunol, Rotterdam, Netherlands4 aut
7001 a Belessi, Chrysoulau Nikea Gen Hosp, Hematol Dept, Piraeus, Greece4 aut
7001 a Davi, Fredericu Hop La Pitie Salpetriere, AP HP, Dept Hematol, Paris, France;UPMC Univ Paris 06, UMRS 1138, Paris, France4 aut
7001 a Rosenquist, Richardu Karolinska Institutet,Uppsala universitet,Science for Life Laboratory, SciLifeLab,Institutionen för immunologi, genetik och patologi,Karolinska Inst, Dept Mol Med & Surg, Stockholm, Sweden4 aut0 (Swepub:uu)richrose
7001 a Stamatopoulos, Kostasu Uppsala universitet,Science for Life Laboratory, SciLifeLab,Institutionen för immunologi, genetik och patologi,Ctr Res & Technol Hellas, Inst Appl Biosci, Thessaloniki, Greece4 aut0 (Swepub:uu)kosst292
7001 a Ghia, Paolou IRCCS Ist Scientifico San Raffaele, Div Expt Oncol, Milan, Italy;Univ Vita Salute San Raffaele, Milan, Italy4 aut
7102 a Karolinska Institutetb Ctr Res & Technol Hellas, Inst Appl Biosci, Thessaloniki, Greece4 org
7730 t Journal of Visualized Experimentsd : JOURNAL OF VISUALIZED EXPERIMENTSg :141q :141x 1940-087X
8564 u https://doi.org/10.3791/57787y Fulltext
8564 u https://uu.diva-portal.org/smash/get/diva2:1287797/FULLTEXT01.pdfx primaryx Raw objecty fulltext:print
8564 u https://www.jove.com/pdf/57787/immunoglobulin-gene-sequence-analysis-chronic-lymphocytic-leukemia
8564 8u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-376902
8564 8u https://doi.org/10.3791/57787
8564 8u http://kipublications.ki.se/Default.aspx?queryparsed=id:140119258

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