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On-chip background ...
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Liu, Zhenhua,1992-Uppsala universitet,Mikrosystemteknik,Science for Life Laboratory, SciLifeLab,EMBLA
(author)
On-chip background dilution in droplets with high particle recovery using acoustophoresis
- Article/chapterEnglish2019
Publisher, publication year, extent ...
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AIP Publishing,2019
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electronicrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:uu-398535
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-398535URI
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https://doi.org/10.1063/1.5129256DOI
Supplementary language notes
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Language:English
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Summary in:English
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Classification
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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Droplet microfluidics has shown great potential for on-chip biological and chemical assays. However, fluid exchange in droplet microfluidics with high particle recovery is still a major bottleneck. Here, using acoustophoresis, we present for the first time a label-free method to achieve continuous background dilution in droplets containing cells with high sample recovery. The system comprises droplet generation, acoustic focusing, droplet splitting, picoinjection, and serpentine mixing on the same chip. The capacities of the picoinjection and the droplet split to dilute the background fluorescent signal in the droplets have been characterized. The sample recovery at different droplet split ratios has also been characterized. The results show a maximum of 4.3-fold background dilution with 87.7% particle recovery. We also demonstrated that the system can be used to dilute background fluorescent signal in droplets containing either polystyrene particles or endothelial cells.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
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Fornell, AnnaUppsala universitet,Mikrosystemteknik,Science for Life Laboratory, SciLifeLab,EMBLA(Swepub:uu)annfo231
(author)
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Barbe, LaurentUppsala universitet,Mikrosystemteknik,Science for Life Laboratory, SciLifeLab,EMBLA(Swepub:uu)lauba497
(author)
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Hjort, Klas,1964-Uppsala universitet,Mikrosystemteknik(Swepub:uu)klashjor
(author)
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Tenje, MariaUppsala universitet,Mikrosystemteknik,Science for Life Laboratory, SciLifeLab,EMBLA(Swepub:uu)marte323
(author)
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Uppsala universitetMikrosystemteknik
(creator_code:org_t)
Related titles
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In:Biomicrofluidics: AIP Publishing131932-1058
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