Sökning: onr:"swepub:oai:DiVA.org:uu-398535" > On-chip background ...
Fältnamn | Indikatorer | Metadata |
---|---|---|
000 | 03143naa a2200385 4500 | |
001 | oai:DiVA.org:uu-398535 | |
003 | SwePub | |
008 | 191206s2019 | |||||||||||000 ||eng| | |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-3985352 URI |
024 | 7 | a https://doi.org/10.1063/1.51292562 DOI |
040 | a (SwePub)uu | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Liu, Zhenhua,d 1992-u Uppsala universitet,Mikrosystemteknik,Science for Life Laboratory, SciLifeLab,EMBLA4 aut0 (Swepub:uu)zheli461 |
245 | 1 0 | a On-chip background dilution in droplets with high particle recovery using acoustophoresis |
264 | 1 | b AIP Publishing,c 2019 |
338 | a electronic2 rdacarrier | |
520 | a Droplet microfluidics has shown great potential for on-chip biological and chemical assays. However, fluid exchange in droplet microfluidics with high particle recovery is still a major bottleneck. Here, using acoustophoresis, we present for the first time a label-free method to achieve continuous background dilution in droplets containing cells with high sample recovery. The system comprises droplet generation, acoustic focusing, droplet splitting, picoinjection, and serpentine mixing on the same chip. The capacities of the picoinjection and the droplet split to dilute the background fluorescent signal in the droplets have been characterized. The sample recovery at different droplet split ratios has also been characterized. The results show a maximum of 4.3-fold background dilution with 87.7% particle recovery. We also demonstrated that the system can be used to dilute background fluorescent signal in droplets containing either polystyrene particles or endothelial cells. | |
650 | 7 | a NATURVETENSKAPx Biologix Biokemi och molekylärbiologi0 (SwePub)106022 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Biochemistry and Molecular Biology0 (SwePub)106022 hsv//eng |
653 | a Teknisk fysik med inriktning mot mikrosystemteknik | |
653 | a Engineering Science with specialization in Microsystems Technology | |
700 | 1 | a Fornell, Annau Uppsala universitet,Mikrosystemteknik,Science for Life Laboratory, SciLifeLab,EMBLA4 aut0 (Swepub:uu)annfo231 |
700 | 1 | a Barbe, Laurentu Uppsala universitet,Mikrosystemteknik,Science for Life Laboratory, SciLifeLab,EMBLA4 aut0 (Swepub:uu)lauba497 |
700 | 1 | a Hjort, Klas,d 1964-u Uppsala universitet,Mikrosystemteknik4 aut0 (Swepub:uu)klashjor |
700 | 1 | a Tenje, Mariau Uppsala universitet,Mikrosystemteknik,Science for Life Laboratory, SciLifeLab,EMBLA4 aut0 (Swepub:uu)marte323 |
710 | 2 | a Uppsala universitetb Mikrosystemteknik4 org |
773 | 0 | t Biomicrofluidicsd : AIP Publishingg 13q 13x 1932-1058 |
856 | 4 | u https://doi.org/10.1063/1.5129256y Fulltext |
856 | 4 | u https://uu.diva-portal.org/smash/get/diva2:1376048/FULLTEXT01.pdfx primaryx Raw objecty fulltext:print |
856 | 4 | u https://aip.scitation.org/doi/pdf/10.1063/1.5129256 |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-398535 |
856 | 4 8 | u https://doi.org/10.1063/1.5129256 |
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