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Sökning: onr:"swepub:oai:DiVA.org:uu-459221" > Wafer-Scale Pattern...

LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00003668naa a2200445 4500
001oai:DiVA.org:uu-459221
003SwePub
008211122s2021 | |||||||||||000 ||eng|
0247 a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-4592212 URI
0247 a https://doi.org/10.3390/mi121113862 DOI
040 a (SwePub)uu
041 a engb eng
042 9 SwePub
072 7a ref2 swepub-contenttype
072 7a art2 swepub-publicationtype
1001 a Kim, Anna Au Uppsala universitet,Mikrosystemteknik,EMBLA4 aut0 (Swepub:uu)annki239
2451 0a Wafer-Scale Patterning of Protein Templates for Hydrogel Fabrication
264 c 2021-11-12
264 1b MDPI,c 2021
338 a electronic2 rdacarrier
500 a Additional fundersThe National Science Foundation (CMMI 1834760). E.A.C. was supported by the National Science Foundation Graduate Research Fellowship (DGE-1656518) and the Ford Foundation Pre-doctoral Fellowship. K.V.L. was supported by the National Science Foundation Graduate Research Fellowship (DGE-1650114). G.V.T. was supported by the Data Driven Biology National Science Foundation Research Traineeship (DGE-2125644).
520 a Human-induced pluripotent stem cell-derived cardiomyocytes are a potentially unlimited cell source and promising patient-specific in vitro model of cardiac diseases. Yet, these cells are limited by immaturity and population heterogeneity. Current in vitro studies aiming at better understanding of the mechanical and chemical cues in the microenvironment that drive cellular maturation involve deformable materials and precise manipulation of the microenvironment with, for example, micropatterns. Such microenvironment manipulation most often involves microfabrication protocols which are time-consuming, require cleanroom facilities and photolithography expertise. Here, we present a method to increase the scale of the fabrication pipeline, thereby enabling large-batch generation of shelf-stable microenvironment protein templates on glass chips. This decreases fabrication time and allows for more flexibility in the subsequent steps, for example, in tuning the material properties and the selection of extracellular matrix or cell proteins. Further, the fabrication of deformable hydrogels has been optimized for compatibility with these templates, in addition to the templates being able to be used to acquire protein patterns directly on the glass chips. With our approach, we have successfully controlled the shapes of cardiomyocytes seeded on Matrigel-patterned hydrogels.
650 7a MEDICIN OCH HÄLSOVETENSKAPx Medicinsk bioteknologi0 (SwePub)3042 hsv//swe
650 7a MEDICAL AND HEALTH SCIENCESx Medical Biotechnology0 (SwePub)3042 hsv//eng
653 a microfabrication; lift-off protein patterning; hydrogels; single-cell cardiomyocytes; single-cell analysis
7001 a Castillo, Erica A.4 aut
7001 a Lane, Kerry V.4 aut
7001 a Torres, Gabriela V.4 aut
7001 a Chirikian, Orlando4 aut
7001 a Wilson, Robin E.4 aut
7001 a Lance, Sydney A.4 aut
7001 a Pardon, Gaspard4 aut
7001 a Pruitt, Beth L.4 aut
7102 a Uppsala universitetb Mikrosystemteknik4 org
7730 t Micromachinesd : MDPIg 12:11, s. 1386-1386q 12:11<1386-1386x 2072-666X
8564 u https://doi.org/10.3390/mi12111386y Fulltext
8564 u https://uu.diva-portal.org/smash/get/diva2:1613309/FULLTEXT01.pdfx primaryx Raw objecty fulltext:print
8564 u https://www.mdpi.com/2072-666X/12/11/1386/pdf
8564 8u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-459221
8564 8u https://doi.org/10.3390/mi12111386

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