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Investigation of neomycin biodegradation conditions using ericoid mycorrhizal and white rot fungal species
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- Stenholm, Åke (författare)
- Uppsala universitet,Analytisk farmaceutisk kemi
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- Hedeland, Mikael (författare)
- Uppsala universitet,Analytisk farmaceutisk kemi
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- Pettersson, Curt (författare)
- Uppsala universitet,Analytisk farmaceutisk kemi
- (creator_code:org_t)
- 2022-10-11
- 2022
- Engelska.
- Ingår i: BMC Biotechnology. - : BMJ Publishing Group Ltd. - 1472-6750. ; 22
- Tidskriftsartikel (refereegranskat)
Abstract
Ämnesord
Stäng
- Background: In the search for methods to biodegrade recalcitrant compounds, the use of saprotrophic fungi and white rot fungi, in particular belonging to the phylum Basidiomycota, has gained interest. This group of fungi possesses a battery of unspecific extracellular enzymes that can be utilized in the biodegradation of preferably phenolic compounds. In this work, it was investigated under which conditions the white rot fungus Trametes versicolor and the ericoid mycorrhizal fungus Rhizoscyphus ericae (belonging to the phylum Ascomycota) could be used to biodegrade the antibiotic aminoglycoside neomycin at co-metabolic conditions in which external nutrients were supplied. Furthermore, it was also investigated whether a biodegradation could be accomplished using neomycin as the sole nutrient.Results: The results show that both species can biodegrade neomycin 70% under co-metabolic conditions during a one-week time course and that Rhizoscyphus ericae is able to use neomycin as sole nutrient and to approximatively biodegrade it 60% under chosen non co-metabolic conditions. At selected conditions, the biodegradation of neomycin using Rhizoscyphus ericae was monitored by oxidation products of D-ribose which is a hydrolysis product of neomycin.Conclusion: The results are of general interest in the search for fungal species that can biodegrade recalcitrant compounds without the need of external nutrients. The key future application area that will be investigated is purification of waste from recombinant protein production in which neomycin, nutrients and E. coli with neomycin resistance genes are present.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- Neomycin
- Biodegradation
- UHPLC-Q-TOF MS
- Trametes versicolor
- Rhizoscyphus ericae
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- Hedeland, Mikael
- Pettersson, Curt
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