onr:"swepub:oai:DiVA.org:uu-498535"
Search: onr:"swepub:oai:DiVA.org:uu-498535" > Bioactive nanocellu...
- 1 of 1
- Previous record
- Next record
- To hitlist
- Blasi Romero, AnnaUppsala universitet,Nanoteknologi och funktionella material (author)
Bioactive nanocellulose materials for the treatment of chronic wounds
- BookEnglish2023
Publisher, publication year, extent ...
- Uppsala :Acta Universitatis Upsaliensis,2023
- 83 s.
- electronicrdacarrier
Numbers
- LIBRIS-ID:oai:DiVA.org:uu-498535
- ISBN:9789151317564
- https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-498535URI
Supplementary language notes
- Language:English
- Summary in:English &language:-1_t
Part of subdatabase
- SwePubSwePub
Classification
Series
- Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology,1651-6214 ;2251
Notes
- Chronic wounds represent a burden for the healthcare system and significantly affect the quality of life of the patients. There is currently a lack of efficient treatments but new, improved therapeutic approaches are under development. Suggested innovative wound care therapies consist on the topical administration of bioactive compounds aimed at restoring the balance in the wound environment and promoting the healing. However, their effectiveness is limited due to the highly oxidative and proteolytic environment in the chronic wound. In the work presented in this thesis, a series of bioactive nanocellulose-based materials were developed with the aim of addressing some of the present demands in chronic wound care. Wood-derived cellulose nanofibrils (CNFs) were functionalized with selected bioactive molecules expected to endow CNFs with the ability to modulate the chronic wound environment. Different chemical approaches were explored to combine CNFs with the following biomolecules: the amino acid cysteine, the peptide oligoproline and the host defense peptide KR-12. Materials were characterized in terms of chemical structure, degree of substitution and bioactivity.The immobilization of cysteine onto CNFs (cys-CNF) provided the material with radical oxygen species (ROS) scavenging properties and the ability to inhibit protease activity, properties that were related to the presence of free thiol groups on the nanofibers. Storage conditions in an inert atmosphere or in the form of aerogel were proposed to assure the long-term activity of the cys-CNF material. Investigations on the use of the ROS-sensitive oligoproline to crosslink CNFs provided optimized protocols to maximize peptide substitution and the degree of crosslinking. The oligoproline-CNF materials were sensitive to ROS-mediated cleavage and provided a protective effect to cells exposed to oxidative conditions. Moreover, the feasibility of preparing ROS-responsive drug delivery hydrogels based on the oligoproline-CNF was demonstrated, with indications that tuning the length of the oligoproline peptide could be exploited to tailor the release rate of small proteins. CNF materials with antibacterial properties and the ability to modulate the response of pro-inflammatory macrophages were obtained by immobilizing KR-12 derivatives onto CNFs. This study highlighted the importance in the selection of the conjugation chemistry to preserve the activity of the peptide once immobilized. To conclude, this work has contributed with valuable strategies to develop bioactive CNF-based materials with the potential of paving the way for advanced solutions in the field of chronic wound care.
Subject headings and genre
- TEKNIK OCH TEKNOLOGIER Nanoteknik hsv//swe
- ENGINEERING AND TECHNOLOGY Nano-technology hsv//eng
- cellulose nanofibrils
- covalent immobilization
- wound healing
- antioxidant properties
- antibacterial properties
- anti-inflammatory properties
- stimuli responsive hydrogels
- reactive oxygen species
- host defense peptides
- Teknisk fysik med inriktning mot nanoteknologi och funktionella material
- Engineering Science with specialization in Nanotechnology and Functional Materials
Added entries (persons, corporate bodies, meetings, titles ...)
- Ferraz, Natalia,Associate Professor,1976-Uppsala universitet,Nanoteknologi och funktionella material(Swepub:uu)natfe727 (thesis advisor)
- Aili, Daniel,ProfessorLinköping University, Department of Physics, Chemistry and Biology, and Department of Biophysics and bioengineering (opponent)
- Uppsala universitetNanoteknologi och funktionella material (creator_code:org_t)
Internet link
Find in a library
- Bioactive nanocellulose materials for the treatment of chronic wounds (Search the publication in LIBRIS)
To the university's database
- 1 of 1
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Blasi Romero, An ...
- Ferraz, Natalia, ...
- Aili, Daniel, Pr ...
- About the subject
-
- ENGINEERING AND TECHNOLOGY
- ENGINEERING AND ...
- and Nano technology
- Parts in the series
- Digital Comprehe ...
- By the university
- Uppsala University
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
