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Sökning: onr:"swepub:oai:DiVA.org:uu-64916" > Application of a co...

LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00003031naa a2200325 4500
001oai:DiVA.org:uu-64916
003SwePub
008081017s2002 | |||||||||||000 ||eng|
0247 a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-649162 URI
0247 a https://doi.org/10.1042/BA200100312 DOI
040 a (SwePub)uu
041 a engb eng
042 9 SwePub
072 7a ref2 swepub-contenttype
072 7a art2 swepub-publicationtype
1001 a Shao, X-W4 aut
2451 0a Application of a colorimetric chain-termination assay for characterization of reverse transcriptase from 3-azido-2,3-deoxythymidine-resistant HIV isolates
264 1c 2002
338 a print2 rdacarrier
520 a wo different enzyme assays, both based on the interaction of native reverse transcriptase(IRT) and 3'-azido-2',3'-deoxythymidine triphosphate (AZT-TP), were used to characterize the enzymesfrom 18 HIV-I isolates with decreased sensitivity to AZT in cell culture. The first assay, which measures the balance between incorporation and excision of AZT monophosphate in the presence of dNTP substrate (in terms of IC50), gave an approx. 9-fold variation in sensitivity to AZT-TP. There was a correlation between the IC50 values and the sensitivity of the corresponding virus to AZT in cell culture (r = 0.60, P < 0.01). The second assay, which was designed specifically for measurement of chain termination in the absence of dNTP substrate (as the concentration of AZT-TP giving 50% residual primer function, or CT50), revealed a more than 600-fold difference between the different isolate RTs. For the majority ofenzymes there was a strict correlation between the results from the two assays; however, four isolatesexhibited significantly higher CT50/IC50 ratios than the other isolates. These differences were not related to sensitivity of the corresponding viruses to AZT but to the occurrence of certain mutations in their pol gene. The four deviating isolates contained either a minimum of four AZT-specific substitutions, including Thr-215 --> Tyr (isolates 134 and 143), or some of the known specific substitutions combined with Thr-39 --> Ala (isolates 80 and 157). The Thr-39 Ala substitution has previously been recorded in connection with AZT/Foscarnet combination therapy.
7001 a Hjalmarsson, Sandrau Uppsala universitet,Klinisk virologi4 aut
7001 a Lennerstrand, Johanu Division of Clinical Virology, Huddinge University Hospital4 aut0 (Swepub:uu)jolen839
7001 a Svennerholm, B4 aut
7001 a Blomberg, Jonasu Uppsala universitet,Klinisk virologi4 aut0 (Swepub:uu)jonablom
7001 a Källander, CFR4 aut
7001 a Gronowitz, J Simon4 aut
7102 a Uppsala universitetb Klinisk virologi4 org
7730 t Biotechnology and applied biochemistryg 35, s. 155-164q 35<155-164x 0885-4513x 1470-8744
8564 8u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-64916
8564 8u https://doi.org/10.1042/BA20010031

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