Two-photon fluorescence correlation microscopy combined with measurements of point spread function; investigations made in human skin

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Fältnamn	Indikatorer	Metadata
000		02652naa a2200289 4500
001		oai:gup.ub.gu.se/133963
003		SwePub
008		240528s2010 \| \|\|\|\|\|\|\|\|\|\|\|000 \|\|eng\|
024	7	a https://gup.ub.gu.se/publication/1339632 URI
040		a (SwePub)gu
041		a eng
042		9 SwePub
072	7	a ref2 swepub-contenttype
072	7	a art2 swepub-publicationtype
100	1	a Guldbrand, Stina,d 1970u Gothenburg University,Göteborgs universitet,Institutionen för fysik (GU),Department of Physics (GU)4 aut0 (Swepub:gu)xgulst
245	1 0	a Two-photon fluorescence correlation microscopy combined with measurements of point spread function; investigations made in human skin
264	1	c 2010
520		a Two-photon excitation fluorescence correlation spectroscopy (TPFCS) has been applied in connection to measurements of the point spread function (PSF) for quantitative analysis of sulphorhodamine B (SRB) in excised human skin. The PSF was measured using subresolution fluorescent beads embedded in the skin specimen. The PSF, measured as full width at half maximum (FWHM) was found to be 0.41 ± 0.05 μm in the lateral direction, and 1.2 ± 0.4 μm in the axial direction. The molecular diffusion of SRB inside the skin ranged between 0.5 and 15.0 × 10 −8 cm2/s. The diffusion coefficient is not dependent on depths down to 40 μm. The fluorophores were found to accumulate on the upper layers of the skin. This work is the first TPFCS study in human skin. The results show that TPFCS can be used for quantitative analyses of fluorescent compounds in human skin.
650	7	a NATURVETENSKAPx Fysikx Atom- och molekylfysik och optik0 (SwePub)103022 hsv//swe
650	7	a NATURAL SCIENCESx Physical Sciencesx Atom and Molecular Physics and Optics0 (SwePub)103022 hsv//eng
700	1	a Simonsson, Carl,d 1976u Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry4 aut0 (Swepub:gu)xsimca
700	1	a Goksör, Mattias,d 1975u Gothenburg University,Göteborgs universitet,Institutionen för fysik (GU),Department of Physics (GU)4 aut0 (Swepub:gu)xgokma
700	1	a Smedh, Maria,d 1968u Gothenburg University,Göteborgs universitet,Institutionen för fysik (GU),Department of Physics (GU)4 aut0 (Swepub:gu)xsmema
700	1	a Ericson, Marica B,d 1974u Gothenburg University,Göteborgs universitet,Institutionen för fysik (GU),Department of Physics (GU)4 aut0 (Swepub:gu)xemarq
710	2	a Göteborgs universitetb Institutionen för fysik (GU)4 org
773	0	t Optics Expressg 18:15, s. 15289-15302q 18:15<15289-15302x 1094-4087
856	4 8	u https://gup.ub.gu.se/publication/133963

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