Sökning: onr:"swepub:oai:gup.ub.gu.se/139836" > RUNX2 Transcription...
Fältnamn | Indikatorer | Metadata |
---|---|---|
000 | 04121naa a2200445 4500 | |
001 | oai:gup.ub.gu.se/139836 | |
003 | SwePub | |
008 | 240528s2010 | |||||||||||000 ||eng| | |
024 | 7 | a https://gup.ub.gu.se/publication/1398362 URI |
040 | a (SwePub)gu | |
041 | a eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Park, Eun-Sil4 aut |
245 | 1 0 | a RUNX2 Transcription Factor Regulates Gene Expression in Luteinizing Granulosa Cells of Rat Ovaries |
264 | 1 | c 2010 |
520 | a The LH surge promotes terminal differentiation of follicular cells to become luteal cells. RUNX2 has been shown to play an important role in cell differentiation, but the regulation of Runx2 expression and its function in the ovary remain to be determined. The present study examined 1) the expression profile of Runx2 and its partner CBFβ during the periovulatory period, 2) regulatory mechanisms of Runx2 expression, and 3) its potential function in the ovary. Runx2 expression was induced in periovulatory granulosa cells of human and rodent ovaries. RUNX2 and core binding factor-β (CBFβ) proteins in nuclear extracts and RUNX2 binding to a consensus binding sequence increased after human chorionic gonadotropin (hCG) administration. This in vivo up-regulation of Runx2 expression was recapitulated in vitro in preovulatory granulosa cells by stimulation with hCG. The hCG-induced Runx2 expression was reduced by antiprogestin (RU486) and EGF-receptor tyrosine kinase inhibitor (AG1478), indicating the involvement of EGF-signaling and progesterone-mediated pathways. We also found that in the C/EBPβ knockout mouse ovary, Runx2 expression was reduced, indicating C/EBPβ-mediated expression. Next, the function of RUNX2 was investigated by suppressing Runx2 expression by small interfering RNA in vitro. Runx2 knockdown resulted in reduced levels of mRNA for Rgc32, Ptgds, Fabp6, Mmp13, and Abcb1a genes. Chromatin immunoprecipitation analysis demonstrated the binding of RUNX2 in the promoter region of these genes, suggesting that these genes are direct downstream targets of RUNX2. Collectively, the present data indicate that the LH surge-induced RUNX2 is involved in various aspects of luteal function by directly regulating the expression of diverse luteal genes. | |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Reproduktionsmedicin och gynekologi0 (SwePub)302202 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Obstetrics, Gynaecology and Reproductive Medicine0 (SwePub)302202 hsv//eng |
653 | a product p-glycoprotein | |
653 | a acid-binding proteins | |
653 | a cellular-localization | |
653 | a hormonal-regulation | |
653 | a tissue inhibitors | |
653 | a ovulatory process | |
653 | a family | |
653 | a receptor | |
653 | a collagenase-3 | |
653 | a oocyte | |
700 | 1 | a Lind, Anna Karin,d 1962u Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för obstetrik och gynekologi,Institute of Clinical Sciences, Department of Obstetrics and Gynecology4 aut0 (Swepub:gu)xlannf |
700 | 1 | a Dahm-Kähler, Pernilla,d 1964u Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för obstetrik och gynekologi,Institute of Clinical Sciences, Department of Obstetrics and Gynecology4 aut0 (Swepub:gu)xdahmp |
700 | 1 | a Brännström, Mats,d 1958u Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för obstetrik och gynekologi,Institute of Clinical Sciences, Department of Obstetrics and Gynecology4 aut0 (Swepub:gu)xbrmat |
700 | 1 | a Carletti, Martha Z.4 aut |
700 | 1 | a Christenson, Lane K.4 aut |
700 | 1 | a Curry, Thomas E.4 aut |
700 | 1 | a Jo, Misung4 aut |
710 | 2 | a Göteborgs universitetb Institutionen för kliniska vetenskaper, Avdelningen för obstetrik och gynekologi4 org |
773 | 0 | t MOLECULAR ENDOCRINOLOGYg 24:4, s. 846-858q 24:4<846-858x 0888-8809 |
856 | 4 8 | u https://gup.ub.gu.se/publication/139836 |
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.