Sökning: onr:"swepub:oai:gup.ub.gu.se/194115" > Real-time PCR thres...
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000 | 04353naa a2200397 4500 | |
001 | oai:gup.ub.gu.se/194115 | |
003 | SwePub | |
008 | 240528s2014 | |||||||||||000 ||eng| | |
009 | oai:prod.swepub.kib.ki.se:128436294 | |
024 | 7 | a https://gup.ub.gu.se/publication/1941152 URI |
024 | 7 | a https://doi.org/10.1128/JCM.02697-132 DOI |
024 | 7 | a http://kipublications.ki.se/Default.aspx?queryparsed=id:1284362942 URI |
040 | a (SwePub)gud (SwePub)ki | |
041 | a eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Elfving, Kristinau Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för pediatrik,Institutionen för biomedicin, avdelningen för infektionssjukdomar,Institute of Clinical Sciences, Department of Pediatrics,Institute of Biomedicine, Department of Infectious Medicine4 aut0 (Swepub:gu)xelfkr |
245 | 1 0 | a Real-time PCR threshold cycle (Ct) cut-offs help to identify agents causing acute childhood diarrhea in Zanzibar. |
264 | 1 | c 2014 |
520 | a Molecular assays might improve identification of causes of acute diarrheal disease, but may lead to more frequent detection of asymptomatic infections. In the present study real-time PCR targeting 14 pathogens was applied on rectal swabs from 330 children aged 2-59 months in Zanzibar, 165 with acute diarrhea and 165 asymptomatic controls. At least one pathogen was detected in 94% of patients and 84% of controls, with higher rates in patients for norovirus genogroup II (20% vs. 2.4%, p<0.0001), rotavirus (10% vs. 1.8%, p=0.003) and Cryptosporidium (30% vs. 11%, p<0.0001). Detection rates did not differ significantly for enterotoxigenic Escherichia coli (ETEC)-estA (33% vs. 24%), ETEC-eltB (44% vs. 46%), Shigella (35% vs. 33%), and Campylobacter (35% vs. 33%), but for these agents Ct (threshold cycle) values were lower (pathogen loads were higher) in sick children than in controls. In multivariate analysis, Ct values for norovirus genogroup II, rotavirus, Cryptosporidium, ETEC-estA and Shigella were independently associated with diarrhea. We conclude that this real-time PCR allows convenient detection of essentially all diarrheagenic agents, and provides Ct values that may be critical for interpretation of results for pathogens with similar detection rates in patients and controls. The results indicate that assessment of pathogen load may improve identification of agents causing gastroenteritis in children. | |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Pediatrik0 (SwePub)302212 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Pediatrics0 (SwePub)302212 hsv//eng |
700 | 1 | a Andersson, Mariau Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för infektionssjukdomar,Institute of Biomedicine, Department of Infectious Medicine4 aut |
700 | 1 | a Msellem, Mwinyi I4 aut |
700 | 1 | a Welinder-Olsson, Christina,d 1959u Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för infektionssjukdomar,Institute of Biomedicine, Department of Infectious Medicine4 aut0 (Swepub:gu)xwelch |
700 | 1 | a Petzold, Max,d 1973u Gothenburg University,Göteborgs universitet,Akademistatistik,Centre for Applied Biostatistics4 aut0 (Swepub:gu)xpemax |
700 | 1 | a Björkman, Andersu Karolinska Institutet4 aut |
700 | 1 | a Trollfors, Birger,d 1947u Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för pediatrik,Institute of Clinical Sciences, Department of Pediatrics4 aut0 (Swepub:gu)xtrobi |
700 | 1 | a Mårtensson, Andreasu Karolinska Institutet4 aut |
700 | 1 | a Lindh, Magnus,d 1960u Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för infektionssjukdomar,Institute of Biomedicine, Department of Infectious Medicine4 aut0 (Swepub:gu)xlmagr |
710 | 2 | a Göteborgs universitetb Institutionen för kliniska vetenskaper, Avdelningen för pediatrik4 org |
773 | 0 | t Journal of clinical microbiologyg 52:3, s. 916-923q 52:3<916-923x 1098-660X |
856 | 4 8 | u https://gup.ub.gu.se/publication/194115 |
856 | 4 8 | u https://doi.org/10.1128/JCM.02697-13 |
856 | 4 8 | u http://kipublications.ki.se/Default.aspx?queryparsed=id:128436294 |
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