Sökning: onr:"swepub:oai:gup.ub.gu.se/219026" > The Unique Non-Cata...
Fältnamn | Indikatorer | Metadata |
---|---|---|
000 | 05829naa a2200589 4500 | |
001 | oai:gup.ub.gu.se/219026 | |
003 | SwePub | |
008 | 240910s2015 | |||||||||||000 ||eng| | |
009 | oai:DiVA.org:his-11448 | |
024 | 7 | a https://gup.ub.gu.se/publication/2190262 URI |
024 | 7 | a https://doi.org/10.1371/journal.pone.01274972 DOI |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:his:diva-114482 URI |
040 | a (SwePub)gud (SwePub)his | |
041 | a eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Ejeskär, Katarina,d 1969u Högskolan i Skövde,Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk genetik och klinisk genetik,Institute of Biomedicine, Department of Medical and Clinical Genetics,Institutionen för biovetenskap,Forskningscentrum för Systembiologi,Department of Medical and Clinical Genetics, Gothenburg University, Gothenburg, Sweden,Tumörbiologi, Tumor Biology4 aut0 (Swepub:his)ejek |
245 | 1 0 | a The Unique Non-Catalytic C-Terminus of P37delta-PI3K Adds Proliferative Properties In Vitro and In Vivo |
264 | c 2015-05-29 | |
264 | 1 | b Public Library of Science (PLoS),c 2015 |
520 | a The PI3K/Akt pathway is central for numerous cellular functions and is frequently deregulated in human cancers. The catalytic subunits of PI3K, p110, are thought to have a potential oncogenic function, and the regulatory subunit p85 exerts tumor suppressor properties. The fruit fly, Drosophila melanogaster, is a highly suitable system to investigate PI3K signaling, expressing one catalytic, Dp110, and one regulatory subunit, Dp60, and both show strong homology with the human PI3K proteins p110 and p85. We recently showed that p37 delta, an alternatively spliced product of human PI3K p110 delta, displayed strong proliferation-promoting properties despite lacking the catalytic domain completely. Here we functionally evaluate the different domains of human p37 delta in Drosophila. The N-terminal region of Dp110 alone promotes cell proliferation, and we show that the unique C-terminal region of human p37 delta further enhances these proliferative properties, both when expressed in Drosophila, and in human HEK-293 cells. Surprisingly, although the N-terminal region of Dp110 and the C-terminal region of p37 delta both display proliferative effects, over-expression of full length Dp110 or the N-terminal part of Dp110 decreases survival in Drosophila, whereas the unique C-terminal region of p37 delta prevents this effect. Furthermore, we found that the N-terminal region of the catalytic subunit of PI3K p110, including only the Dp60 (p85)-binding domain and a minor part of the Ras binding domain, rescues phenotypes with severely impaired development caused by Dp60 over-expression in Drosophila, possibly by regulating the levels of Dp60, and also by increasing the levels of phosphorylated Akt. Our results indicate a novel kinase-independent function of the PI3K catalytic subunit. | |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicin0 (SwePub)3022 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Clinical Medicine0 (SwePub)3022 hsv//eng |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Medicinsk genetik0 (SwePub)301072 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Medical Genetics0 (SwePub)301072 hsv//eng |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Cell- och molekylärbiologi0 (SwePub)301082 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Cell and Molecular Biology0 (SwePub)301082 hsv//eng |
653 | a DROSOPHILA PHOSPHOINOSITIDE 3-KINASE | |
653 | a LIFE-SPAN | |
653 | a PHOSPHATIDYLINOSITOL | |
653 | a 3-KINASE | |
653 | a NEUROBLASTOMA TUMORS | |
653 | a P110 DELTA | |
653 | a FAT-BODY | |
653 | a GROWTH | |
653 | a EXPRESSION | |
653 | a SUBUNIT | |
653 | a PTEN | |
653 | a Medical sciences | |
700 | 1 | a Vickes, O.u Högskolan i Skövde,Forskningscentrum för Systembiologi4 aut |
700 | 1 | a Kuchipudi, A.u Högskolan i Skövde,Forskningscentrum för Systembiologi4 aut |
700 | 1 | a Wettergren, Yvonne,d 1957u Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för kirurgi,Institute of Clinical Sciences, Department of Surgery,Department of General Surgery, Gothenburg University, Gothenburg, Sweden4 aut0 (Swepub:gu)xwetyv |
700 | 1 | a Uv, Anne,d 1967u Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk genetik och klinisk genetik,Institute of Biomedicine, Department of Medical and Clinical Genetics,Department of Medical and Clinical Genetics, Gothenburg University, Gothenburg, Sweden4 aut0 (Swepub:gu)xuvanz |
700 | 1 | a Rotter Sopasakis, Victoria,d 1972u Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för molekylär och klinisk medicin,Institute of Medicine, Department of Molecular and Clinical Medicine,Department of Molecular and Clinical Medicine, Institute of Medicine, Wallenberg Laboratory, Gothenburg University, Gothenburg, Sweden4 aut0 (Swepub:gu)xrotvi |
710 | 2 | a Göteborgs universitetb Institutionen för biomedicin, avdelningen för medicinsk genetik och klinisk genetik4 org |
773 | 0 | t Plos Oned : Public Library of Science (PLoS)g 10:5q 10:5x 1932-6203 |
856 | 4 | u https://doi.org/10.1371/journal.pone.0127497 |
856 | 4 | u https://doi.org/10.1371/journal.pone.0127497y Fulltext |
856 | 4 8 | u https://gup.ub.gu.se/publication/219026 |
856 | 4 8 | u https://doi.org/10.1371/journal.pone.0127497 |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:his:diva-11448 |
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