onr:"swepub:oai:gup.ub.gu.se/313565"
Sökning: onr:"swepub:oai:gup.ub.gu.se/313565" > CDKN2C expression i...
- 1 av 1
- Föregående post
- Nästa post
- Till träfflistan
CDKN2C expression in adipose tissue is reduced in type II diabetes and central obesity: impact on adipocyte differentiation and lipid storage?
-
- Pereira, Maria J., 1981- (författare)
- Uppsala universitet,Klinisk diabetologi och metabolism
-
- Vranic, Milica (författare)
- Uppsala universitet,Klinisk diabetologi och metabolism
-
- Kamble, Prasad G. (författare)
- Uppsala universitet,Klinisk diabetologi och metabolism
- visa fler...
- visa färre...
- (creator_code:org_t)
- Elsevier BV, 2022
- 2022
- Engelska.
- Ingår i: Translational Research. - : Elsevier BV. - 1931-5244 .- 1878-1810. ; 242, s. 105-121
- Tidskriftsartikel (refereegranskat)
Abstract
Ämnesord
Stäng
- CDKN2C/p18 (Cyclin-Dependent Kinase Inhibitor 2C) is a cell growth regulator that controls cell cycle progression and has previously been associated with increased risk for type II diabetes (T2D) and reduced peripheral adipose tissue (AT) storage capacity. This study explored the role of CDKN2C in AT lipid and glucose metabolism in T2D. Expression of CDKN2C and other genes was analyzed by transcriptomics, or real-time PCR in subcutaneous AT (SAT) samples obtained from T2D and control subjects matched for sex, age and BMI and also in paired SAT and omental AT (OAT) samples. Functional studies included adipocyte glucose uptake and lipolysis rates. CRISPR/Cas9 CDKN2C gene knockdown was performed in human preadipocytes to assess adipogenesis. CDKN2C mRNA expression in SAT and OAT was reduced in T2D and obese subjects compared to controls. CDKN2C expression in SAT was inversely correlated with measures of hyperglycemia, insulin resistance and visceral adiposity and positively correlated with expression of genes in several metabolic pathways, including insulin signaling and fatty acid and carbohydrate metabolism. CDKN2C protein was mainly expressed in adipocytes compared to stromal vascular cells, and its gene and protein expression was up-regulated during adipocyte differentiation. Knockdown of CDKN2C did not affect the percentage of differentiating cells compared to wild type cultures. However, CDKN2C knockdown cultures had significantly lower expression of differentiation markers CEBPA, ADIPOQ and FASN and transiently reduced lipid accumulation per adipocyte during differentiation. Our findings suggest that adipose CDKN2C expression might be reduced as a consequence of insulin resistance and obesity, and this can further contribute to impairment of SAT lipid storage. © 2021 The Authors
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Endokrinologi och diabetes (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Endocrinology and Diabetes (hsv//eng)
Nyckelord
- ADIPOQ=Adiponectin
- AT=Adipose tissue
- CDKN2C=Cyclin-Dependent Kinase Inhibitor 2C
- CEBPA=CCAAT Enhancer Binding Protein Alpha
- FASN=Fatty Acid Synthase
- GWAS=Genome-wide association study
- MRI=Magnetic resonance imaging
- OAT=Omental adipose tissue
- PPARG=Peroxisome Proliferator-Activated Receptor Gamma
- RNP=Ribonucleoprotein
- SAT=Subcutaneous adipose tissue
- sgRNA=Single-guide RNA
- SVF=Stromal vascular fraction
- T2D=Type II diabetes
- VAT=Visceral adipose tissue
- WHR=Waist-hip ratio
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
- 1 av 1
- Föregående post
- Nästa post
- Till träfflistan
Hitta mer i SwePub
- Av författaren/redakt...
- Pereira, Maria J ...
- Vranic, Milica
- Kamble, Prasad G ...
- Jernow, Henning
- Kristofi, Robin
- Holbikova, E. M. ...
- visa fler...
- Skrtic, Stanko, ...
- Kullberg, Joel, ...
- Svensson, Maria ...
- Hetty, Susanne, ...
- Eriksson, Jan
- visa färre...
- Om ämnet
-
- MEDICIN OCH HÄLSOVETENSKAP
- MEDICIN OCH HÄLS ...
- och Klinisk medicin
- och Endokrinologi oc ...
- Artiklar i publikationen
- Translational Re ...
- Av lärosätet
- Göteborgs universitet
- Uppsala universitet
Sök utanför SwePub
- Sök vidare i:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - Nationella bibliotekssystem
- LIBRIS.kb.se
