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Vesicle Collision P...
Vesicle Collision Protocols for the Study of Quantum Size and Exocytotic Fraction Released
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- Majdi, Soodabeh, 1980 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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- Da Silva Lima, Alex (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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- Ewing, Andrew G, 1957 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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(creator_code:org_t)
- 2022-10-08
- 2023
- Engelska.
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Ingår i: Methods in Molecular Biology. - New York, NY : Springer US. - 1064-3745 .- 1940-6029. ; , s. 223-237
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- We review the methods of vesicle impact electrochemical cytometry, intracellular impact electrochemical cytometry, and single cell amperometry and their application to measuring the storage of neurotransmitters in cellular vesicles. We provide protocols to measure vesicle content, the release of catecholamines, and from there the fraction of transmitter released in each exocytosis event. The focus here has been a combination of methods to evaluate factors related to neuronal function at the cellular level and implications in, for example, cognition.
Ämnesord
- NATURVETENSKAP -- Kemi -- Oorganisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Inorganic Chemistry (hsv//eng)
Nyckelord
- Electrochemical cytometry
- Electrochemistry
- Exocytosis
- Neurotransmitters
- Vesicles
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