Sökning: onr:"swepub:oai:lup.lub.lu.se:156fab3c-b199-42d0-9da6-f4ad1bdf4537" > Multicenter prospec...
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000 | 05394naa a2200661 4500 | |
001 | oai:lup.lub.lu.se:156fab3c-b199-42d0-9da6-f4ad1bdf4537 | |
003 | SwePub | |
008 | 230130s2023 | |||||||||||000 ||eng| | |
009 | oai:prod.swepub.kib.ki.se:151326630 | |
024 | 7 | a https://lup.lub.lu.se/record/156fab3c-b199-42d0-9da6-f4ad1bdf45372 URI |
024 | 7 | a https://doi.org/10.1002/cyto.b.221052 DOI |
024 | 7 | a http://kipublications.ki.se/Default.aspx?queryparsed=id:1513266302 URI |
040 | a (SwePub)lud (SwePub)ki | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a art2 swepub-publicationtype |
072 | 7 | a ref2 swepub-contenttype |
100 | 1 | a Kern, Wolfgangu Munich Leukemia Laboratory (MLL)4 aut |
245 | 1 0 | a Multicenter prospective evaluation of diagnostic potential of flow cytometric aberrancies in myelodysplastic syndromes by the ELN iMDS flow working group |
264 | c 2022-11-23 | |
264 | 1 | b Wiley,c 2023 |
300 | a 15 s. | |
520 | a Background: Myelodysplastic syndromes (MDS) represent a diagnostic challenge. This prospective multicenter study was conducted to evaluate pre-defined flow cytometric markers in the diagnostic work-up of MDS and chronic myelomonocytic leukemia (CMML). Methods: Thousand six hundred and eighty-two patients with suspected MDS/CMML were analyzed by both cytomorphology according to WHO 2016 criteria and flow cytometry according to ELN recommendations. Flow cytometric readout was categorized ‘non-MDS’ (i.e. no signs of MDS/CMML and limited signs of MDS/CMML) and ‘in agreement with MDS’ (i.e., in agreement with MDS/CMML). Results: Flow cytometric readout categorized 60% of patients in agreement with MDS, 28% showed limited signs of MDS and 12% had no signs of MDS. In 81% of cases flow cytometric readouts and cytomorphologic diagnosis correlated. For high-risk MDS, the level of concordance was 92%. A total of 17 immunophenotypic aberrancies were found independently related to MDS/CMML in ≥1 of the subgroups of low-risk MDS, high-risk MDS, CMML. A cut-off of ≥3 of these aberrancies resulted in 80% agreement with cytomorphology (20% cases concordantly negative, 60% positive). Moreover, >3% myeloid progenitor cells were significantly associated with MDS (286/293 such cases, 98%). Conclusion: Data from this prospective multicenter study led to recognition of 17 immunophenotypic markers allowing to identify cases ‘in agreement with MDS’. Moreover, data emphasizes the clinical utility of immunophenotyping in MDS diagnostics, given the high concordance between cytomorphology and the flow cytometric readout. Results from the current study challenge the application of the cytomorphologically defined cut-off of 5% blasts for flow cytometry and rather suggest a 3% cut-off for the latter. | |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Hematologi0 (SwePub)302022 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Hematology0 (SwePub)302022 hsv//eng |
653 | a flow cytometry | |
653 | a hematology | |
653 | a multiparameter analysis | |
653 | a myelodysplastic syndrome | |
700 | 1 | a Westers, Theresia M.u Amsterdam UMC - Vrije Universiteit Amsterdam4 aut |
700 | 1 | a Bellos, Fraukeu Munich Leukemia Laboratory (MLL)4 aut |
700 | 1 | a Bene, Marie Christineu Nantes University Hospital4 aut |
700 | 1 | a Bettelheim, Peteru Elisabethinen Hospital Linz4 aut |
700 | 1 | a Brodersen, Lisa Eidenschinku Hematologics Inc.4 aut |
700 | 1 | a Burbury, Kateu Peter MacCallum Cancer Centre4 aut |
700 | 1 | a Chu, Sung Chao4 aut |
700 | 1 | a Cullen, Matthewu St James's University Hospital4 aut |
700 | 1 | a Porta, Matteo Dellau Humanitas Research Hospital4 aut |
700 | 1 | a Dunlop, Alan Stewartu UCL Cancer Institute4 aut |
700 | 1 | a Johansson, Ulrikau University Hospitals Bristol NHS Foundation Trust4 aut |
700 | 1 | a Matarraz, Sergiou University of Salamanca4 aut |
700 | 1 | a Oelschlaegel, Utau Dresden University of Technology4 aut |
700 | 1 | a Ogata, Kiyoyuki4 aut |
700 | 1 | a Porwit, Annau Lund University,Lunds universitet,Patologi, Lund,Sektion V,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Pathology, Lund,Section V,Department of Clinical Sciences, Lund,Faculty of Medicine4 aut0 (Swepub:lu)an6742po |
700 | 1 | a Preijers, Franku Radboud University Medical Center4 aut |
700 | 1 | a Psarra, Katherinau Evangelismos Athens General Hospital4 aut |
700 | 1 | a Saft, Leonieu Karolinska Institutet,Karolinska University Hospital4 aut |
700 | 1 | a Subirá, Doloresu Hospital Universitario de Guadalajara4 aut |
700 | 1 | a Weiß, Elisabethu Munich Leukemia Laboratory (MLL)4 aut |
700 | 1 | a van der Velden, Vincent H.J.u Erasmus University Medical Center4 aut |
700 | 1 | a van de Loosdrecht, Arjanu Amsterdam UMC - Vrije Universiteit Amsterdam4 aut |
700 | 1 | a Weiss, E4 aut |
710 | 2 | a Munich Leukemia Laboratory (MLL)b Amsterdam UMC - Vrije Universiteit Amsterdam4 org |
773 | 0 | t Cytometry Part B - Clinical Cytometryd : Wileyg 104:1, s. 51-65q 104:1<51-65x 1552-4949x 1552-4957 |
856 | 4 | u http://dx.doi.org/10.1002/cyto.b.22105y FULLTEXT |
856 | 4 8 | u https://lup.lub.lu.se/record/156fab3c-b199-42d0-9da6-f4ad1bdf4537 |
856 | 4 8 | u https://doi.org/10.1002/cyto.b.22105 |
856 | 4 8 | u http://kipublications.ki.se/Default.aspx?queryparsed=id:151326630 |
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