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Sökning: onr:"swepub:oai:lup.lub.lu.se:632a07b8-3f33-4b73-b2ca-b11c480f3093" > Monitoring the inte...

LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00005049naa a2200433 4500
001oai:lup.lub.lu.se:632a07b8-3f33-4b73-b2ca-b11c480f3093
003SwePub
008220307s2022 | |||||||||||000 ||eng|
0247 a https://lup.lub.lu.se/record/632a07b8-3f33-4b73-b2ca-b11c480f30932 URI
0247 a https://doi.org/10.1038/s41598-022-06846-92 DOI
040 a (SwePub)lu
041 a engb eng
042 9 SwePub
072 7a art2 swepub-publicationtype
072 7a ref2 swepub-contenttype
1001 a Torres-Garcia, Laurau Lund University,Lunds universitet,Experimentell demensforskning,Forskargrupper vid Lunds universitet,Neural plasticitet och reparation,Experimental Dementia Research,Lund University Research Groups,Neural Plasticity and Repair4 aut0 (Swepub:lu)la6204to
2451 0a Monitoring the interactions between alpha-synuclein and Tau in vitro and in vivo using bimolecular fluorescence complementation
264 c 2022-02-22
264 1b Springer Science and Business Media LLC,c 2022
520 a Parkinson’s disease (PD) and Alzheimer’s disease (AD) are characterized by pathological accumulation and aggregation of different amyloidogenic proteins, α-synuclein (aSyn) in PD, and amyloid-β (Aβ) and Tau in AD. Strikingly, few PD and AD patients’ brains exhibit pure pathology with most cases presenting mixed types of protein deposits in the brain. Bimolecular fluorescence complementation (BiFC) is a technique based on the complementation of two halves of a fluorescent protein, which allows direct visualization of protein–protein interactions. In the present study, we assessed the ability of aSyn and Tau to interact with each other. For in vitro evaluation, HEK293 and human neuroblastoma cells were used, while in vivo studies were performed by AAV6 injection in the substantia nigra pars compacta (SNpc) of mice and rats. We observed that the co-expression of aSyn and Tau led to the emergence of fluorescence, reflecting the interaction of the proteins in cell lines, as well as in mouse and rat SNpc. Thus, our data indicates that aSyn and Tau are able to interact with each other in a biologically relevant context, and that the BiFC assay is an effective tool for studying aSyn-Tau interactions in vitro and in different rodent models in vivo.
650 7a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Neurovetenskaper0 (SwePub)301052 hsv//swe
650 7a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Neurosciences0 (SwePub)301052 hsv//eng
7001 a Domingues, Joana M. P.u Lund University4 aut
7001 a Brandi, Edoardou Lund University,Lunds universitet,Neural plasticitet och reparation,Forskargrupper vid Lunds universitet,Neural Plasticity and Repair,Lund University Research Groups4 aut0 (Swepub:lu)ed2621br
7001 a Haikal, Carolineu Lund University,Lunds universitet,Neural plasticitet och reparation,Forskargrupper vid Lunds universitet,Neural Plasticity and Repair,Lund University Research Groups4 aut0 (Swepub:lu)ca6087ha
7001 a Mudannayake, Janitha M.u Lund University,Lunds universitet,Utvecklings- och regenerativ neurobiologi,Forskargrupper vid Lunds universitet,Developmental and Regenerative Neurobiology,Lund University Research Groups4 aut0 (Swepub:lu)ja6032mu
7001 a Brás, Inês C.u University Medical Center Göttingen4 aut
7001 a Gerhardt, Ellenu University Medical Center Göttingen4 aut
7001 a Li, Wenu Lund University,Lunds universitet,Neural plasticitet och reparation,Forskargrupper vid Lunds universitet,Neural Plasticity and Repair,Lund University Research Groups,China Medical University, Shenyang4 aut0 (Swepub:lu)med-wnl
7001 a Svanbergsson, Alexanderu Lund University,Lunds universitet,Neural plasticitet och reparation,Forskargrupper vid Lunds universitet,Neural Plasticity and Repair,Lund University Research Groups4 aut0 (Swepub:lu)med-as29
7001 a Outeiro, Tiago F.u Max Planck Institute of Experimental Medicine,University of Newcastle upon Tyne,University Medical Center Göttingen4 aut
7001 a Gouras, Gunnar K.u Lund University,Lunds universitet,Experimentell demensforskning,Forskargrupper vid Lunds universitet,Experimental Dementia Research,Lund University Research Groups4 aut0 (Swepub:lu)med-grg
7001 a Li, Jia Yiu Lund University,Lunds universitet,Neural plasticitet och reparation,Forskargrupper vid Lunds universitet,Neural Plasticity and Repair,Lund University Research Groups,China Medical University, Shenyang4 aut0 (Swepub:lu)mphy-jli
7102 a Experimentell demensforskningb Forskargrupper vid Lunds universitet4 org
7730 t Scientific Reportsd : Springer Science and Business Media LLCg 12, s. 1-11q 12<1-11x 2045-2322
8564 u http://dx.doi.org/10.1038/s41598-022-06846-9x freey FULLTEXT
8564 u https://www.nature.com/articles/s41598-022-06846-9.pdf
8564 8u https://lup.lub.lu.se/record/632a07b8-3f33-4b73-b2ca-b11c480f3093
8564 8u https://doi.org/10.1038/s41598-022-06846-9

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