Sökning: onr:"swepub:oai:lup.lub.lu.se:85680bf1-bb96-40ee-b201-72750692b2de" > Nordström Ina > Long-term effects o...
Fältnamn | Indikatorer | Metadata |
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000 | 04537naa a2200433 4500 | |
001 | oai:lup.lub.lu.se:85680bf1-bb96-40ee-b201-72750692b2de | |
003 | SwePub | |
008 | 160401s1999 | |||||||||||000 ||eng| | |
024 | 7 | a https://lup.lub.lu.se/record/11152582 URI |
040 | a (SwePub)lu | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a art2 swepub-publicationtype |
072 | 7 | a ref2 swepub-contenttype |
100 | 1 | a Lindqvist, Andersu Lund University,Lunds universitet,Islet cell physiology,Forskargrupper vid Lunds universitet,Lund University Research Groups4 aut0 (Swepub:lu)mphy-ali |
245 | 1 0 | a Long-term effects of Ca(2+) on structure and contractility of vascular smooth muscle |
264 | 1 | c 1999 |
520 | a Culture of dispersed smooth muscle cells is known to cause rapid modulation from the contractile to the synthetic cellular phenotype. However, organ culture of smooth muscle tissue, with maintained extracellular matrix and cell-cell contacts, may facilitate maintenance of the contractile phenotype. To test the influence of culture conditions, structural, functional, and biochemical properties of rat tail arterial rings were investigated after culture. Rings were cultured for 4 days in the absence and presence of 10% FCS and then mounted for physiological experiments. Intracellular Ca(2+) concentration ([Ca(2+)](i)) after stimulation with norepinephrine was similar in rings cultured with and without FCS, whereas force development after FCS was decreased by >50%. The difference persisted after permeabilization with beta-escin. These effects were associated with the presence of vasoconstrictors in FCS and were dissociated from its growth-stimulatory action. FCS treatment increased lactate production but did not affect ATP, ADP, or AMP contents. The contents of actin and myosin were decreased by culture but similar for all culture conditions. There was no effect of FCS on calponin contents or myosin SM1/SM2 isoform composition, nor was there any appearance of nonmuscle myosin. FCS-stimulated rings showed evidence of cell degeneration not found after culture without FCS or with FCS + verapamil (1 microM) to lower [Ca(2+)](i). The decreased force-generating ability after culture with FCS is thus associated with increased [Ca(2+)](i) during culture and not primarily caused by growth-associated modulation of cells from the contractile to the synthetic phenotype. | |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Endokrinologi och diabetes0 (SwePub)302052 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Endocrinology and Diabetes0 (SwePub)302052 hsv//eng |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Läkemedelskemi0 (SwePub)301032 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Medicinal Chemistry0 (SwePub)301032 hsv//eng |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Farmakologi och toxikologi0 (SwePub)301022 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Pharmacology and Toxicology0 (SwePub)301022 hsv//eng |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Fysiologi0 (SwePub)301062 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Physiology0 (SwePub)301062 hsv//eng |
653 | a tail artery | |
653 | a organ culture | |
653 | a contraction | |
653 | a calcium | |
653 | a ultrastructure | |
700 | 1 | a Nordström, Inau Lund University,Lunds universitet,Kärlfysiologi,Forskargrupper vid Lunds universitet,Vascular Physiology,Lund University Research Groups4 aut0 (Swepub:lu)mphy-ino |
700 | 1 | a Malmqvist, Ulfu Lund University,Lunds universitet,Avdelningen för klinisk kemi och farmakologi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Clinical Chemistry and Pharmacology,Department of Laboratory Medicine,Faculty of Medicine4 aut0 (Swepub:lu)mphy-uma |
700 | 1 | a Nordenfelt, Patrik4 aut |
700 | 1 | a Hellstrand, Peru Lund University,Lunds universitet,Kärlfysiologi,Forskargrupper vid Lunds universitet,Vascular Physiology,Lund University Research Groups4 aut0 (Swepub:lu)mphy-phe |
710 | 2 | a Islet cell physiologyb Forskargrupper vid Lunds universitet4 org |
773 | 0 | t American Journal of Physiology: Cell Physiologyg 277:1, s. 64-73q 277:1<64-73x 1522-1563 |
856 | 4 | u http://ajpcell.physiology.org/cgi/content/full/277/1/C64y FULLTEXT |
856 | 4 8 | u https://lup.lub.lu.se/record/1115258 |
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