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Sökning: onr:"swepub:oai:lup.lub.lu.se:9a579750-8d30-43e4-b6d6-f8267e2ee4a9" > Hepatocyte-like cel...

LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00003904naa a2200493 4500
001oai:lup.lub.lu.se:9a579750-8d30-43e4-b6d6-f8267e2ee4a9
003SwePub
008160401s2010 | |||||||||||000 ||eng|
009oai:prod.swepub.kib.ki.se:120086259
0247 a https://lup.lub.lu.se/record/15681392 URI
0247 a https://doi.org/10.1016/j.jbiotec.2009.11.0072 DOI
0247 a http://kipublications.ki.se/Default.aspx?queryparsed=id:1200862592 URI
040 a (SwePub)lud (SwePub)ki
041 a engb eng
042 9 SwePub
072 7a art2 swepub-publicationtype
072 7a ref2 swepub-contenttype
1001 a Brolen, Gabriella4 aut
2451 0a Hepatocyte-like cells derived from human embryonic stem cells specifically via definitive endoderm and a progenitor stage
264 1b Elsevier BV,c 2010
520 a Human embryonic stem cells offer a potential unlimited supply for functional hepatocytes, since they can differentiate into hepatocyte-like cells displaying a characteristic hepatic morphology and expressing various hepatic markers. These cells could be used in various applications such as studies of drug metabolism and hepatotoxicity, which however, would require a significant expression of drug metabolizing enzymes. To derive these cells we use a stepwise differentiation protocol where growth- and maturation factors are added. The first phase involves the formation of definitive endoderm. Next, these cells are treated with factors known to promote the induction and proliferation towards hepatic progenitor cell types. In the last phase the cells are terminally differentiated and maturated into functional hepatocyte-like cells. The cultures were characterized by analysis of endodermal or hepatic markers and compared to cultures derived without induction via definitive endoderm. Hepatic functions such as urea secretion, glycogen storage, indocyanine green uptake and secretion, and cytochrome P450-expression and activity were evaluated. The DE-Hep showed a hepatocyte morphology with sub-organized cells and exhibited many liver-functions including transporter activity and capacity to metabolize drugs specific for important cytochrome P450 sub-families. This represents an importantstep in differentiation of hESC into functional hepatocytes. (C) 2009 Elsevier B.V. All rights reserved.
650 7a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Cell- och molekylärbiologi0 (SwePub)301082 hsv//swe
650 7a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Cell and Molecular Biology0 (SwePub)301082 hsv//eng
653 a Liver
653 a Hepatocyte
653 a Human embryonic stem cell
653 a Definitive endoderm
653 a progenitor
653 a Activin
7001 a Sivertsson, Louiseu Karolinska Institutet4 aut
7001 a Bjorquist, Petter4 aut
7001 a Eriksson, Gustav4 aut
7001 a Ek, Monica4 aut
7001 a Semb, Henriku Lund University,Lunds universitet,Stamcellscentrum (SCC),Avdelningen för stamcellsforskning,Institutionen för laboratoriemedicin,Medicinska fakulteten,Stem Cell Center,Division of stem cell research,Department of Laboratory Medicine,Faculty of Medicine4 aut0 (Swepub:lu)endo-hse
7001 a Johansson, Ingeru Karolinska Institutet4 aut
7001 a Andersson, Tommy B.4 aut
7001 a Ingelman-Sundberg, Magnusu Karolinska Institutet4 aut
7001 a Heins, Nico4 aut
7102 a Karolinska Institutetb Stamcellscentrum (SCC)4 org
7730 t Journal of Biotechnologyd : Elsevier BVg 145:3, s. 284-294q 145:3<284-294x 1873-4863x 0168-1656
8564 u http://dx.doi.org/10.1016/j.jbiotec.2009.11.007y FULLTEXT
8564 8u https://lup.lub.lu.se/record/1568139
8564 8u https://doi.org/10.1016/j.jbiotec.2009.11.007
8564 8u http://kipublications.ki.se/Default.aspx?queryparsed=id:120086259

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