Sökning: onr:"swepub:oai:lup.lub.lu.se:9a579750-8d30-43e4-b6d6-f8267e2ee4a9" > Hepatocyte-like cel...
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000 | 03904naa a2200493 4500 | |
001 | oai:lup.lub.lu.se:9a579750-8d30-43e4-b6d6-f8267e2ee4a9 | |
003 | SwePub | |
008 | 160401s2010 | |||||||||||000 ||eng| | |
009 | oai:prod.swepub.kib.ki.se:120086259 | |
024 | 7 | a https://lup.lub.lu.se/record/15681392 URI |
024 | 7 | a https://doi.org/10.1016/j.jbiotec.2009.11.0072 DOI |
024 | 7 | a http://kipublications.ki.se/Default.aspx?queryparsed=id:1200862592 URI |
040 | a (SwePub)lud (SwePub)ki | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a art2 swepub-publicationtype |
072 | 7 | a ref2 swepub-contenttype |
100 | 1 | a Brolen, Gabriella4 aut |
245 | 1 0 | a Hepatocyte-like cells derived from human embryonic stem cells specifically via definitive endoderm and a progenitor stage |
264 | 1 | b Elsevier BV,c 2010 |
520 | a Human embryonic stem cells offer a potential unlimited supply for functional hepatocytes, since they can differentiate into hepatocyte-like cells displaying a characteristic hepatic morphology and expressing various hepatic markers. These cells could be used in various applications such as studies of drug metabolism and hepatotoxicity, which however, would require a significant expression of drug metabolizing enzymes. To derive these cells we use a stepwise differentiation protocol where growth- and maturation factors are added. The first phase involves the formation of definitive endoderm. Next, these cells are treated with factors known to promote the induction and proliferation towards hepatic progenitor cell types. In the last phase the cells are terminally differentiated and maturated into functional hepatocyte-like cells. The cultures were characterized by analysis of endodermal or hepatic markers and compared to cultures derived without induction via definitive endoderm. Hepatic functions such as urea secretion, glycogen storage, indocyanine green uptake and secretion, and cytochrome P450-expression and activity were evaluated. The DE-Hep showed a hepatocyte morphology with sub-organized cells and exhibited many liver-functions including transporter activity and capacity to metabolize drugs specific for important cytochrome P450 sub-families. This represents an importantstep in differentiation of hESC into functional hepatocytes. (C) 2009 Elsevier B.V. All rights reserved. | |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Cell- och molekylärbiologi0 (SwePub)301082 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Cell and Molecular Biology0 (SwePub)301082 hsv//eng |
653 | a Liver | |
653 | a Hepatocyte | |
653 | a Human embryonic stem cell | |
653 | a Definitive endoderm | |
653 | a progenitor | |
653 | a Activin | |
700 | 1 | a Sivertsson, Louiseu Karolinska Institutet4 aut |
700 | 1 | a Bjorquist, Petter4 aut |
700 | 1 | a Eriksson, Gustav4 aut |
700 | 1 | a Ek, Monica4 aut |
700 | 1 | a Semb, Henriku Lund University,Lunds universitet,Stamcellscentrum (SCC),Avdelningen för stamcellsforskning,Institutionen för laboratoriemedicin,Medicinska fakulteten,Stem Cell Center,Division of stem cell research,Department of Laboratory Medicine,Faculty of Medicine4 aut0 (Swepub:lu)endo-hse |
700 | 1 | a Johansson, Ingeru Karolinska Institutet4 aut |
700 | 1 | a Andersson, Tommy B.4 aut |
700 | 1 | a Ingelman-Sundberg, Magnusu Karolinska Institutet4 aut |
700 | 1 | a Heins, Nico4 aut |
710 | 2 | a Karolinska Institutetb Stamcellscentrum (SCC)4 org |
773 | 0 | t Journal of Biotechnologyd : Elsevier BVg 145:3, s. 284-294q 145:3<284-294x 1873-4863x 0168-1656 |
856 | 4 | u http://dx.doi.org/10.1016/j.jbiotec.2009.11.007y FULLTEXT |
856 | 4 8 | u https://lup.lub.lu.se/record/1568139 |
856 | 4 8 | u https://doi.org/10.1016/j.jbiotec.2009.11.007 |
856 | 4 8 | u http://kipublications.ki.se/Default.aspx?queryparsed=id:120086259 |
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