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Sökning: onr:"swepub:oai:lup.lub.lu.se:ae5029b5-8db7-4b55-abe3-5443451c69ca" > Fractional 13C enri...

LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00003922naa a2200481 4500
001oai:lup.lub.lu.se:ae5029b5-8db7-4b55-abe3-5443451c69ca
003SwePub
008160401s2007 | |||||||||||000 ||eng|
0247 a https://lup.lub.lu.se/record/5724652 URI
0247 a https://doi.org/10.1007/s10858-007-9158-62 DOI
040 a (SwePub)lu
041 a engb eng
042 9 SwePub
072 7a art2 swepub-publicationtype
072 7a ref2 swepub-contenttype
1001 a Lundström, Patriku Lund University,Lunds universitet,Biofysikalisk kemi,Centrum för Molekylär Proteinvetenskap,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biophysical Chemistry,Center for Molecular Protein Science,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH4 aut0 (Swepub:lu)molm-plu
2451 0a Fractional 13C enrichment of isolated carbons using [1-13C]- or [2-13C]-glucose facilitates the accurate measurement of dynamics at backbone Ca and side-chain methyl positions in protein
264 c 2007-06-07
264 1b Springer Science and Business Media LLC,c 2007
520 a A simple labeling approach is presented based on protein expression in [1-C-13]- or [2-C-13]-glucose containing media that produces molecules enriched at methyl carbon positions or backbone C-alpha sites, respectively. All of the methyl groups, with the exception of Thr and Ile(delta 1) are produced with isolated C-13 spins (i.e., no C-13-C-13 one bond couplings), facilitating studies of dynamics through the use of spin-spin relaxation experiments without artifacts introduced by evolution due to large homonuclear scalar couplings. Carbon-alpha sites are labeled without concomitant labeling at C-beta positions for 17 of the common 20 amino acids and there are no cases for which C-13(alpha)-(CO)-C-13 spin pairs are observed. A large number of probes are thus available for the study of protein dynamics with the results obtained complimenting those from more traditional backbone N-15 studies. The utility of the labeling is established by recording C-13 R-1 rho and CPMG-based experiments on a number of different protein systems.
650 7a NATURVETENSKAPx Kemix Fysikalisk kemi0 (SwePub)104022 hsv//swe
650 7a NATURAL SCIENCESx Chemical Sciencesx Physical Chemistry0 (SwePub)104022 hsv//eng
653 a [1-C-13]-glucose
653 a protein expression
653 a selective C-13 labeling
653 a dispersion
653 a CPMG relaxation
653 a T1 rho
653 a C-13 relaxation measurements
653 a [2-C-13]-glucose
7001 a Teilum, Kaareu Lund University,Lunds universitet,Biofysikalisk kemi,Centrum för Molekylär Proteinvetenskap,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biophysical Chemistry,Center for Molecular Protein Science,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH4 aut0 (Swepub:lu)bpc-kte
7001 a Carstensen, Tommy4 aut
7001 a Bezsonova, Irina4 aut
7001 a Wiesner, Silke4 aut
7001 a Hansen, D. Flemming4 aut
7001 a Religa, Tomasz L.4 aut
7001 a Akke, Mikaelu Lund University,Lunds universitet,Biofysikalisk kemi,Centrum för Molekylär Proteinvetenskap,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biophysical Chemistry,Center for Molecular Protein Science,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH4 aut0 (Swepub:lu)fkm2-mak
7001 a Kay, Lewis E.4 aut
7102 a Biofysikalisk kemib Centrum för Molekylär Proteinvetenskap4 org
7730 t Journal of Biomolecular NMRd : Springer Science and Business Media LLCg 38:3, s. 199-212q 38:3<199-212x 1573-5001x 0925-2738
8564 u http://dx.doi.org/10.1007/s10858-007-9158-6y FULLTEXT
8564 8u https://lup.lub.lu.se/record/572465
8564 8u https://doi.org/10.1007/s10858-007-9158-6

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