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Sökning: onr:"swepub:oai:lup.lub.lu.se:b4e54f7e-b764-46e0-9e39-9677889b69d3" > Chondroitin sulfate...

LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00004547naa a2200481 4500
001oai:lup.lub.lu.se:b4e54f7e-b764-46e0-9e39-9677889b69d3
003SwePub
008160401s2008 | |||||||||||000 ||eng|
0247 a https://lup.lub.lu.se/record/12056192 URI
0247 a https://doi.org/10.1634/stemcells.2007-08982 DOI
040 a (SwePub)lu
041 a engb eng
042 9 SwePub
072 7a art2 swepub-publicationtype
072 7a ref2 swepub-contenttype
1001 a Singhal, Shweta4 aut
2451 0a Chondroitin sulfate proteoglycans and microglia prevent migration and integration of grafted Muller stem cells into degenerating retina
264 c 2008-01-24
264 1b Oxford University Press (OUP),c 2008
520 a At present, there are severe limitations to the successful migration and integration of stem cells transplanted into the degenerated retina to restore visual function. This study investigated the potential role of chondroitin sulfate proteoglycans (CSPGs) and microglia in the migration of human Muller glia with neural stem cell characteristics following subretinal injection into the Lister hooded (LH) and Royal College of Surgeons (RCS) rat retinae. Neonate LH rat retina showed minimal baseline microglial accumulation (CD68-positive cells) that increased significantly 2 weeks after transplantation (p <.001), particularly in the ganglion cell layer (GCL) and inner plexiform layer. In contrast, nontransplanted 5-week-old RCS rat retina showed considerable baseline microglial accumulation in the outer nuclear layer (ONL) and photoreceptor outer segment debris zone (DZ) that further increased (p <.05) throughout the retina 2 weeks after transplantation. Marked deposition of the N-terminal fragment of CSPGs, as well as neurocan and versican, was observed in the DZ of 5-week-old RCS rat retinae, which contrasted with the limited expression of these proteins in the GCL of the adult and neonate LH rat retinae. Staining for CSPGs and CD68 revealed colocalization of these two molecules in cells infiltrating the ONL and DZ of the degenerating RCS rat retina. Enhanced immune suppression with oral prednisolone and intraperitoneal injections of indomethacin caused a reduction in the number of microglia but did not facilitate Muller stem cell migration. However, injection of cells with chondroitinase ABC combined with enhanced immune suppression caused a dramatic increase in the migration of Muller stem cells into all the retinal cell layers. These observations suggest that both microglia and CSPGs constitute a barrier for stem cell migration following transplantation into experimental models of retinal degeneration and that control of matrix deposition and the innate microglial response to neural retina degeneration may need to be addressed when translating cell-based therapies to treat human retinal disease.
650 7a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Cell- och molekylärbiologi0 (SwePub)301082 hsv//swe
650 7a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Cell and Molecular Biology0 (SwePub)301082 hsv//eng
653 a tissue regeneration tissue-specific stem cells
653 a experimental models
653 a stem cell transplantation
653 a adult stem cells
653 a cell migration
7001 a Lawrence, Jean M4 aut
7001 a Bhatia, Bhairavi4 aut
7001 a Ellis, James S4 aut
7001 a Kwan, Anthony S4 aut
7001 a MacNeil, Angus4 aut
7001 a Luthert, Philip J4 aut
7001 a Fawcett, James W4 aut
7001 a Perez, Maria Therezau Lund University,Lunds universitet,Oftalmologi, Lund,Sektion IV,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Nanomaterial för retinala implantat,Forskargrupper vid Lunds universitet,Ophthalmology, Lund,Section IV,Department of Clinical Sciences, Lund,Faculty of Medicine,Nanomaterials for retinal prostheses,Lund University Research Groups4 aut0 (Swepub:lu)oft-mtp
7001 a Khaw, Peng T4 aut
7001 a Limb, Astrid4 aut
7102 a Oftalmologi, Lundb Sektion IV4 org
7730 t Stem Cellsd : Oxford University Press (OUP)g 26:4, s. 1074-1082q 26:4<1074-1082x 1549-4918x 1066-5099
8564 u http://dx.doi.org/10.1634/stemcells.2007-0898y FULLTEXT
8564 u https://stemcellsjournals.onlinelibrary.wiley.com/doi/pdfdirect/10.1634/stemcells.2007-0898
8564 8u https://lup.lub.lu.se/record/1205619
8564 8u https://doi.org/10.1634/stemcells.2007-0898

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