onr:"swepub:oai:lup.lub.lu.se:b7f31d5c-c018-47b0-b2c2-d0d213391824"
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| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 04767naa a2200433 4500 | |
| 001 | oai:lup.lub.lu.se:b7f31d5c-c018-47b0-b2c2-d0d213391824 | |
| 003 | SwePub | |
| 008 | 160401s2006 | |||||||||||000 ||eng| | |
| 024 | 7 | a https://lup.lub.lu.se/record/4140122 URI |
| 024 | 7 | a https://doi.org/10.1021/pr050354v2 DOI |
| 040 | a (SwePub)lu | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a art2 swepub-publicationtype |
| 072 | 7 | a ref2 swepub-contenttype |
| 100 | 1 | a Alm, Rikardu Lund University,Lunds universitet,Biokemi och Strukturbiologi,Centrum för Molekylär Proteinvetenskap,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biochemistry and Structural Biology,Center for Molecular Protein Science,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH4 aut0 (Swepub:lu)med-ral |
| 245 | 1 0 | a Detection and identification of protein isoforms using cluster analysis of MALDI-MS mass spectra |
| 264 | c 2006-03-16 | |
| 264 | 1 | b American Chemical Society (ACS),c 2006 |
| 520 | a We describe an approach to screen large sets of MALDI-MS mass spectra for protein isoforms separated on two-dimensional electrophoresis gels. Mass spectra are matched against each other by utilizing extracted peak mass lists and hierarchical clustering. The output is presented as dendrograms in which protein isoforms cluster together. Clustering could be applied to mass spectra from different sample sets, dates, and instruments, revealed similarities between mass spectra, and was a useful tool to highlight peptide peaks of interest for further investigation. Shared peak masses in a cluster could be identified and were used to create novel peak mass lists suitable for protein identification using peptide mass fingerprinting. Complex mass spectra consisting of more than one protein were deconvoluted using information from other mass spectra in the same cluster. The number of peptide peaks shared between mass spectra in a cluster was typically found to be larger than the number of peaks that matched to calculated peak masses in databases, thus modified peaks are probably among the shared peptides. Clustering increased the number of peaks associated with a given protein. | |
| 650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Cancer och onkologi0 (SwePub)302032 hsv//swe |
| 650 | 7 | a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Cancer and Oncology0 (SwePub)302032 hsv//eng |
| 653 | a proteomics | |
| 653 | a protein | |
| 653 | a hierarchical clustering | |
| 653 | a identification | |
| 653 | a isoforms | |
| 653 | a mass spectra | |
| 700 | 1 | a Johansson, Peteru Lund University,Lunds universitet,Beräkningsbiologi och biologisk fysik - Genomgår omorganisation,Institutionen för astronomi och teoretisk fysik - Genomgår omorganisation,Naturvetenskapliga fakulteten,Computational Biology and Biological Physics - Undergoing reorganization,Department of Astronomy and Theoretical Physics - Undergoing reorganization,Faculty of Science4 aut0 (Swepub:lu)thep-pjo |
| 700 | 1 | a Hjerno, K4 aut |
| 700 | 1 | a Emanuelsson, Ceciliau Lund University,Lunds universitet,Biokemi och Strukturbiologi,Centrum för Molekylär Proteinvetenskap,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biochemistry and Structural Biology,Center for Molecular Protein Science,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH4 aut0 (Swepub:lu)biok-cem |
| 700 | 1 | a Ringnér, Markusu Lund University,Lunds universitet,Beräkningsbiologi och biologisk fysik - Genomgår omorganisation,Institutionen för astronomi och teoretisk fysik - Genomgår omorganisation,Naturvetenskapliga fakulteten,Computational Biology and Biological Physics - Undergoing reorganization,Department of Astronomy and Theoretical Physics - Undergoing reorganization,Faculty of Science4 aut0 (Swepub:lu)thep-mri |
| 700 | 1 | a Häkkinen, Jariu Lund University,Lunds universitet,Beräkningsbiologi och biologisk fysik - Genomgår omorganisation,Institutionen för astronomi och teoretisk fysik - Genomgår omorganisation,Naturvetenskapliga fakulteten,Computational Biology and Biological Physics - Undergoing reorganization,Department of Astronomy and Theoretical Physics - Undergoing reorganization,Faculty of Science4 aut0 (Swepub:lu)thep-jha |
| 710 | 2 | a Biokemi och Strukturbiologib Centrum för Molekylär Proteinvetenskap4 org |
| 773 | 0 | t Journal of Proteome Researchd : American Chemical Society (ACS)g 5:4, s. 785-792q 5:4<785-792x 1535-3893x 1535-3907 |
| 856 | 4 | u http://dx.doi.org/10.1021/pr050354vy FULLTEXT |
| 856 | 4 | u http://cbbp.thep.lu.se/pub/Preprints/05/lu_tp_05_20.pdf |
| 856 | 4 8 | u https://lup.lub.lu.se/record/414012 |
| 856 | 4 8 | u https://doi.org/10.1021/pr050354v |
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