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| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 04469naa a2200385 4500 | |
| 001 | oai:lup.lub.lu.se:bcbb7fce-2856-4e6e-857e-657fffe43a66 | |
| 003 | SwePub | |
| 008 | 160404s2010 | |||||||||||000 ||eng| | |
| 024 | 7 | a https://lup.lub.lu.se/record/15952652 URI |
| 024 | 7 | a https://doi.org/10.1007/s10620-010-1202-92 DOI |
| 040 | a (SwePub)lu | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a art2 swepub-publicationtype |
| 072 | 7 | a ref2 swepub-contenttype |
| 100 | 1 | a Feng, Danu Lund University,Lunds universitet,Medicin, Lund,Sektion II,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Medicine, Lund,Section II,Department of Clinical Sciences, Lund,Faculty of Medicine4 aut0 (Swepub:lu)med-dfg |
| 245 | 1 0 | a Generating Ceramide from Sphingomyelin by Alkaline Sphingomyelinase in the Gut Enhances Sphingomyelin-Induced Inhibition of Cholesterol Uptake in Caco-2 Cells. |
| 264 | c 2010-04-16 | |
| 264 | 1 | b Springer Science and Business Media LLC,c 2010 |
| 338 | a electronic2 rdacarrier | |
| 520 | a BACKGROUND: Sphingomyelin (SM) is present in dietary products and cell plasma membranes. We previously showed that dietary SM inhibited cholesterol absorption in rats. In the intestinal tract, SM is mainly hydrolyzed by alkaline sphingomyelinase (alk-SMase) to ceramide. AIMS: We investigated the influence of SM and its hydrolytic products ceramide and sphingosine on cholesterol uptake in intestinal Caco-2 cells. METHODS: Micelles containing bile salt, monoolein, and (14)C-cholesterol were prepared with or without SM, ceramide, or sphingosine. The micelles were incubated with Caco-2 cells, and uptake of radioactive cholesterol was quantified. RESULTS: We found that confluent monolayer Caco-2 cells expressed NPC1L1, and the uptake of cholesterol in the cells was inhibited by ezetimibe, a specific inhibitor of NPC1L1. Incorporation of SM in the cholesterol micelles inhibited cholesterol uptake dose-dependently; 38% inhibition occurred at an equal mole ratio of SM and cholesterol. The inhibition was further enhanced to 45% by pretreating the cholesterol/SM micelles with recombinant alk-SMase, which hydrolyzed SM in the micelles by 85%, indicating ceramide has stronger inhibitory effects on cholesterol uptake. To confirm this, we further replaced SM in the micelles with ceramide and sphingosine, and found that at equal mole ratio to cholesterol, ceramide exhibited stronger inhibitory effect (50% vs 38%) on cholesterol uptake than SM, whereas sphingosine only had a weak effect at high concentrations. CONCLUSION: Both SM and ceramide inhibit cholesterol uptake, the effect of ceramide being stronger than that of SM. Alk-SMase enhances SM-induced inhibition of cholesterol uptake by generating ceramide in the intestinal lumen. | |
| 650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Gastroenterologi0 (SwePub)302132 hsv//swe |
| 650 | 7 | a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Gastroenterology and Hepatology0 (SwePub)302132 hsv//eng |
| 700 | 1 | a Ohlsson, Lenau Lund University,Lunds universitet,Medicin, Lund,Sektion II,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Medicine, Lund,Section II,Department of Clinical Sciences, Lund,Faculty of Medicine4 aut0 (Swepub:lu)med-loh |
| 700 | 1 | a Ling, Wenhua4 aut |
| 700 | 1 | a Nilsson, Åkeu Lund University,Lunds universitet,Medicin, Lund,Sektion II,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Medicine, Lund,Section II,Department of Clinical Sciences, Lund,Faculty of Medicine4 aut0 (Swepub:lu)med-ani |
| 700 | 1 | a Duan, Rui-Dongu Lund University,Lunds universitet,Medicin, Lund,Sektion II,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Medicine, Lund,Section II,Department of Clinical Sciences, Lund,Faculty of Medicine4 aut0 (Swepub:lu)med-rdd |
| 710 | 2 | a Medicin, Lundb Sektion II4 org |
| 773 | 0 | t Digestive Diseases and Sciencesd : Springer Science and Business Media LLCg Mar 4, s. 3377-3383q Mar 4<3377-3383x 1573-2568x 0163-2116 |
| 856 | 4 | u https://portal.research.lu.se/files/5181290/1637889.pdfx primaryx freey FULLTEXT |
| 856 | 4 | u http://www.ncbi.nlm.nih.gov/pubmed/20393874?dopt=Abstracty FULLTEXT |
| 856 | 4 | u http://dx.doi.org/10.1007/s10620-010-1202-9y FULLTEXT |
| 856 | 4 | u https://lup.lub.lu.se/search/files/5181290/1637889.pdf |
| 856 | 4 8 | u https://lup.lub.lu.se/record/1595265 |
| 856 | 4 8 | u https://doi.org/10.1007/s10620-010-1202-9 |
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