Sökning: onr:"swepub:oai:lup.lub.lu.se:c143e026-d2c8-4761-919f-2f36fbdf5c03" > Effects of ions on ...
Fältnamn | Indikatorer | Metadata |
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000 | 04233naa a2200397 4500 | |
001 | oai:lup.lub.lu.se:c143e026-d2c8-4761-919f-2f36fbdf5c03 | |
003 | SwePub | |
008 | 160401s1996 | |||||||||||000 ||eng| | |
024 | 7 | a https://lup.lub.lu.se/record/1262812 URI |
024 | 7 | a https://doi.org/10.1016/0304-4165(96)00031-12 DOI |
040 | a (SwePub)lu | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a art2 swepub-publicationtype |
072 | 7 | a ref2 swepub-contenttype |
100 | 1 | a Johansson, Hans-Olofu Lund University,Lunds universitet,Biokemi och Strukturbiologi,Centrum för Molekylär Proteinvetenskap,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biochemistry and Structural Biology,Center for Molecular Protein Science,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH4 aut0 (Swepub:lu)biok-hoj |
245 | 1 0 | a Effects of ions on partitioning of serum albumin and lysozyme in aqueous two-phase systems containing ethylene oxide/propylene oxide co-polymers |
264 | 1 | b Elsevier BV,c 1996 |
520 | a Aqueous two-phase systems composed of ethylene oxide/propylene oxide random co-polymers, EO30/PO70 or Ucon (EO50/PO50), in the top phase and dextran T500 in the bottom phase, have been studied. The cloud point diagram for EO30/PO70 in water solution was determined. EO30/PO70 has a cloud point of 32oC at a concentration of 10% (w/w). The phase diagram for the system EO30/PO70-dextran T500-water was determined. Salt effects have been studied on the partitioning of two model proteins, bovine serum albumin and hen egg white lysozyme, in EO30/PO70-dextran and Ucon-dextran systems. Ions with different hydrophobicity, i.e., with different position in the Hofmeister or lyotropic series, were investigated with reference to their effect on protein partition. The counterion hydrophobicity was shown to have a strong influence on the partitioning of BSA and lysozyme. Most extreme partitioning was obtained with hydrophobic (chaotropic) ions like ClO-4 and I-. A comparison of protein partitioning between PEG-dextran and EO30/PO70-dextran has been done. A more extreme protein partitioning was obtained in the EO30/PO70-dextran containing system. Temperature-induced phase separation was studied with EO30/PO70 at 45oC. Both BSA and lysozyme were completely partitioned to the water phase formed above the cloud point of EO30/PO70. Model calculations, based on Flory-Huggins theory of polymer solutions, have been done which could reproduce the salt effect on the protein partitioning in aqueous-two phase system. | |
650 | 7 | a NATURVETENSKAPx Biologi0 (SwePub)1062 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Biological Sciences0 (SwePub)1062 hsv//eng |
650 | 7 | a NATURVETENSKAPx Kemix Teoretisk kemi0 (SwePub)104072 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Chemical Sciencesx Theoretical Chemistry0 (SwePub)104072 hsv//eng |
653 | a Polymer | |
653 | a Phase separation | |
653 | a Partitioning of protein | |
653 | a Aqueous two-phase system | |
653 | a Temperature-induced phase separation | |
700 | 1 | a Lundh, Gabriella4 aut |
700 | 1 | a Karlström, Gunnaru Lund University,Lunds universitet,Beräkningskemi,Enheten för fysikalisk och teoretisk kemi,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Computational Chemistry,Physical and theoretical chemistry,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH4 aut0 (Swepub:lu)teok-gka |
700 | 1 | a Tjerneld, Folkeu Lund University,Lunds universitet,Biokemi och Strukturbiologi,Centrum för Molekylär Proteinvetenskap,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biochemistry and Structural Biology,Center for Molecular Protein Science,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH4 aut0 (Swepub:lu)biok-ftj |
710 | 2 | a Biokemi och Strukturbiologib Centrum för Molekylär Proteinvetenskap4 org |
773 | 0 | t Biochimica et Biophysica Acta. General Subjectsd : Elsevier BVg 1290:3, s. 289-298q 1290:3<289-298x 0304-4165 |
856 | 4 | u http://dx.doi.org/10.1016/0304-4165(96)00031-1y FULLTEXT |
856 | 4 8 | u https://lup.lub.lu.se/record/126281 |
856 | 4 8 | u https://doi.org/10.1016/0304-4165(96)00031-1 |
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