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Enhanced survival of porcine neural xenografts in mice lacking CD1d1, but no effect of NK1.1 depletion
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- Larsson, L C (author)
- Lund University,Lunds universitet,Neurobiologi,Forskargrupper vid Lunds universitet,Neurobiology,Lund University Research Groups
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- Anderson, P (author)
- Lund University
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- Widner, H (author)
- Lund University,Lunds universitet,Neurologi, Lund,Sektion IV,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Regeneration in Movement Disorders,Forskargrupper vid Lunds universitet,Neurology, Lund,Section IV,Department of Clinical Sciences, Lund,Faculty of Medicine,Lund University Research Groups
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- Korsgrent, O (author)
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- (creator_code:org_t)
- 2017-06-22
- 2001
- English 10 s.
- In: Cell Transplantation. - : SAGE Publications. - 0963-6897 .- 1555-3892. ; 10:3, s. 295-304
- Journal article (peer-reviewed)
Abstract
Subject headings
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- Transplantation of embryonic porcine neurons may restore neurological function in patients with Parkinson's disease, if immunological rejection could be prevented. This study was performed to investigate the role of natural killer cells (NK cells) and NK1.1+ T cells (NK T cells) in the rejection of neural xenografts. A cell suspension was prepared from the ventral mesencephalon of 26-27-day-old pig embryos, and 2 microl was implanted in the right striata of mutant CD1d1 null (CD1.1-/-) mice, NK1.1-depleted mice, and controls. The CD1.1-/- mice are deficient in NK T cells and the antigen-presenting molecule CD1d1. Graft survival and host responses were determined immunohistochemically using markers for dopamine neurons, CD4-, CD8- cells, microglia, and macrophages. At 2 weeks, the grafts were significantly larger in CD1.1-/- mice, 0.09 +/- 0.02 microl (mean +/- SEM), compared with controls, 0.05 +/- 0.01 microl. There was no significant difference between NK1.1-depleted mice, 0.02 +/- 0.01 microl, and controls. At 5 weeks, two grafts were still present in the CD1-/- mice, whereas only scars remained in the controls and in the NK1.1-depleted mice. Immune reactions were strong at 2 weeks and less pronounced at 5 weeks in all groups. Microglial activation was lower in NK-depleted mice than in the controls at 2 weeks. In contrast to organ xenografting, NK1.1+ cells do not seem to be important mediators of the rejection of discordant cellular neural xenografts. However, our results suggest that the antigen-presenting molecule CD1d1 may be involved in the rejection process.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- Animals
- Antigens, CD1
- Antigens, CD1d
- Brain Tissue Transplantation
- CD4-Positive T-Lymphocytes
- CD8-Positive T-Lymphocytes
- Fetal Tissue Transplantation
- Flow Cytometry
- Graft Survival
- Killer Cells, Natural
- Macrophages
- Mice
- Mice, Inbred C57BL
- Mice, Mutant Strains
- Microglia
- Swine
- Transplantation, Heterologous
- Journal Article
- Research Support, Non-U.S. Gov't
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- art (subject category)
- ref (subject category)
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- Larsson, L C
- Anderson, P
- Widner, H
- Korsgrent, O
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