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Sökning: onr:"swepub:oai:lup.lub.lu.se:e6c3c7bc-e945-4ef0-a609-ec29c2b1f038" > Selective targeting...

LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00003455naa a2200373 4500
001oai:lup.lub.lu.se:e6c3c7bc-e945-4ef0-a609-ec29c2b1f038
003SwePub
008180924s2017 | |||||||||||000 ||eng|
0247 a https://lup.lub.lu.se/record/e6c3c7bc-e945-4ef0-a609-ec29c2b1f0382 URI
0247 a https://doi.org/10.1126/sciadv.17004882 DOI
040 a (SwePub)lu
041 a engb eng
042 9 SwePub
072 7a art2 swepub-publicationtype
072 7a ref2 swepub-contenttype
1001 a Aprile, Francesco A.u University of Cambridge4 aut
2451 0a Selective targeting of primary and secondary nucleation pathways in Ab42 aggregation using a rational antibody scanning method
264 1b American Association for the Advancement of Science (AAAS),c 2017
520 a Antibodies targeting Ab42 are under intense scrutiny because of their therapeutic potential for Alzheimer’s disease. To enable systematic searches, we present an “antibody scanning” strategy for the generation of a panel of antibodies against Ab42. Each antibody in the panel is rationally designed to target a specific linear epitope, with the selected epitopes scanning the Ab42 sequence. By screening in vitro the panel to identify the specific microscopic steps in the Ab42 aggregation process influenced by each antibody, we identify two antibodies that target specifically the primary and the secondary nucleation steps, which are key for the production of Ab42 oligomers. These two antibodies act, respectively, to delay the onset of aggregation and to block the proliferation of aggregates, and correspondingly reduce the toxicity in a Caenorhabditis elegans model over-expressing Ab42. These results illustrate how the antibody scanning method described here can be used to readily obtain very small antibody libraries with extensive coverage of the sequences of target proteins.
650 7a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Cell- och molekylärbiologi0 (SwePub)301082 hsv//swe
650 7a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Cell and Molecular Biology0 (SwePub)301082 hsv//eng
7001 a Sormanni, Pietrou University of Cambridge4 aut
7001 a Perni, Micheleu University of Cambridge4 aut
7001 a Arosio, Paolou ETH Zürich4 aut
7001 a Linse, Sarau Lund University,Lunds universitet,NanoLund: Centre for Nanoscience,Annan verksamhet, LTH,Lunds Tekniska Högskola,Biokemi och Strukturbiologi,Centrum för Molekylär Proteinvetenskap,Kemiska institutionen,Institutioner vid LTH,Other operations, LTH,Faculty of Engineering, LTH,Biochemistry and Structural Biology,Center for Molecular Protein Science,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH4 aut0 (Swepub:lu)fkm2-sli
7001 a Knowles, Tuomas P.J.u University of Cambridge4 aut
7001 a Dobson, Christopher M.u University of Cambridge4 aut
7001 a Vendruscolo, Micheleu University of Cambridge4 aut
7102 a University of Cambridgeb ETH Zürich4 org
7730 t Science Advancesd : American Association for the Advancement of Science (AAAS)g 3:6q 3:6x 2375-2548
8564 u http://dx.doi.org/10.1126/sciadv.1700488x freey FULLTEXT
8564 u https://advances.sciencemag.org/content/advances/3/6/e1700488.full.pdf
8564 8u https://lup.lub.lu.se/record/e6c3c7bc-e945-4ef0-a609-ec29c2b1f038
8564 8u https://doi.org/10.1126/sciadv.1700488

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