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Sökning: onr:"swepub:oai:lup.lub.lu.se:eeb8b68f-1c51-4546-a892-2093c2c54927" > A Novel Capacitive ...

LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00004186naa a2200361 4500
001oai:lup.lub.lu.se:eeb8b68f-1c51-4546-a892-2093c2c54927
003SwePub
008180813s2018 | |||||||||||000 ||eng|
0247 a https://lup.lub.lu.se/record/eeb8b68f-1c51-4546-a892-2093c2c549272 URI
0247 a https://doi.org/10.1016/j.bios.2018.07.0702 DOI
040 a (SwePub)lu
041 a engb eng
042 9 SwePub
072 7a art2 swepub-publicationtype
072 7a ref2 swepub-contenttype
1001 a Canfarotta, Francescou University of Leicester4 aut
2451 0a A Novel Capacitive Sensor Based on Molecularly Imprinted Nanoparticles as Recognition Elements
264 1b Elsevier BV,c 2018
520 a Molecularly Imprinted Polymers (MIPs) are synthetic receptors capable of selective binding to their target (template) molecules and, hence, are used as recognition elements in assays and sensors as a replacement for relatively unstable enzymes and antibodies. Herein, we describe a manufacturing-friendly protocol for integration of MIP nanoparticles (nanoMIPs) with a (label-free) capacitive sensor. The nanoMIPs were produced by solid-phase synthesis for two templates with different sizes and properties, including a small molecule tetrahydrocannabinol (THC) and a protein (trypsin). NanoMIPs were deposited on the surface of the sensor and the change in capacitance (ΔC) upon binding of the target was measured. The significant improvement in the selectivity and limit of detection (one order of magnitude compared to previously used MIP microparticles) can be attributed to their increased surface-to-volume ratio and higher specificity of the nanoMIPs produced by the solid-phase method. The methodology described is also compatible with common sensor fabrication approaches, as opposed to methods involving in situ MIP polymerisation. The proposed sensor shows high selectivity, fast sensor response (45 min including injection, regeneration and re-equilibration with running buffer), and straightforward data analysis, which makes it viable for label-free monitoring in real-time. The set of targets assessed in this manuscript shows the general applicability of the biosensor platform.
650 7a MEDICIN OCH HÄLSOVETENSKAPx Medicinsk bioteknologix Medicinsk bioteknologi0 (SwePub)304012 hsv//swe
650 7a MEDICAL AND HEALTH SCIENCESx Medical Biotechnologyx Medical Biotechnology0 (SwePub)304012 hsv//eng
7001 a Czulak, Joannau University of Leicester4 aut
7001 a Guerreiro, Antoniou University of Leicester4 aut
7001 a Cruz, Alvaro Garciau University of Leicester4 aut
7001 a Piletsky, Sergeyu University of Leicester4 aut
7001 a Ertürk Bergdahl, Gizemu Lund University,Lunds universitet,Infektionsmedicin,Sektion III,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Experimental Infection Medicine,Forskargrupper vid Lunds universitet,Infection Medicine (BMC),Section III,Department of Clinical Sciences, Lund,Faculty of Medicine,Lund University Research Groups,CapSenze Biosystems AB4 aut0 (Swepub:lu)gi2856er
7001 a Hedström, Martinu Lund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH,CapSenze Biosystems AB4 aut0 (Swepub:lu)biot-mhe
7001 a Mattiasson, Bou Lund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH,CapSenze Biosystems AB4 aut0 (Swepub:lu)biot-bma
7102 a University of Leicesterb Infektionsmedicin4 org
7730 t Biosensors and Bioelectronicsd : Elsevier BVg , s. 108-114q <108-114x 0956-5663
8564 u http://dx.doi.org/10.1016/j.bios.2018.07.070y FULLTEXT
8564 8u https://lup.lub.lu.se/record/eeb8b68f-1c51-4546-a892-2093c2c54927
8564 8u https://doi.org/10.1016/j.bios.2018.07.070

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