Sökning: onr:"swepub:oai:lup.lub.lu.se:f6cd482a-13ae-450a-a437-2cfcafb9e503" >
Tezepelumab decreas...
Tezepelumab decreases airway epithelial IL-33 and T2-inflammation in response to viral stimulation in patients with asthma
-
- Sverrild, A. (författare)
- Bispebjerg Hospital
-
- Cerps, S. (författare)
- Lund University,Lunds universitet,Respiratorisk immunofarmakologi,Forskargrupper vid Lunds universitet,Respiratory Immunopharmacology,Lund University Research Groups
-
- Nieto-Fontarigo, J. J. (författare)
- Lund University,Lunds universitet,Respiratorisk immunofarmakologi,Forskargrupper vid Lunds universitet,Respiratory Immunopharmacology,Lund University Research Groups,CIBER de Fisiopatología Obesidad y Nutricion (CIBEROBN),University of Santiago de Compostela,Health Research Institute of Santiago de Compostela (IDIS)
-
visa fler...
-
- Ramu, S. (författare)
- Lund University,Lunds universitet,Respiratorisk immunofarmakologi,Forskargrupper vid Lunds universitet,Respiratory Immunopharmacology,Lund University Research Groups
-
- Hvidtfeldt, M. (författare)
- Bispebjerg Hospital
-
- Menzel, M. (författare)
- Lund University,Lunds universitet,Respiratorisk immunofarmakologi,Forskargrupper vid Lunds universitet,Respiratory Immunopharmacology,Lund University Research Groups
-
- Kearley, J. (författare)
- AstraZeneca, UK
-
- Griffiths, J. M. (författare)
- AstraZeneca, UK
-
- Parnes, J. R. (författare)
- Amgen Inc.
-
- Porsbjerg, C. (författare)
- Bispebjerg Hospital
-
- Uller, L. (författare)
- Lund University,Lunds universitet,Institutionen för experimentell medicinsk vetenskap,Medicinska fakulteten,Respiratorisk immunofarmakologi,Forskargrupper vid Lunds universitet,Department of Experimental Medical Science,Faculty of Medicine,Respiratory Immunopharmacology,Lund University Research Groups
-
visa färre...
-
(creator_code:org_t)
- 2024
- 2024
- Engelska 12 s.
-
Ingår i: Allergy: European Journal of Allergy and Clinical Immunology. - 0105-4538. ; 79:3, s. 656-666
- Relaterad länk:
-
http://dx.doi.org/10... (free)
-
visa fler...
-
https://lup.lub.lu.s...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- Background: Respiratory virus infections are main triggers of asthma exacerbations. Tezepelumab, an anti-TSLP mAb, reduces exacerbations in patients with asthma, but the effect of blocking TSLP on host epithelial resistance and tolerance to virus infection is not known. Aim: To examine effects of blocking TSLP in patients with asthma on host resistance (IFNβ, IFNλ, and viral load) and on the airway epithelial inflammatory response to viral challenge. Methods: Bronchoalveolar lavage fluid (BALF, n = 39) and bronchial epithelial cells (BECs) were obtained from patients with uncontrolled asthma before and after 12 weeks of tezepelumab treatment (n = 13) or placebo (n = 13). BECs were cultured in vitro and exposed to the viral infection mimic poly(I:C) or infected by rhinovirus (RV). Alarmins, T2- and pro-inflammatory cytokines, IFNβ IFNλ, and viral load were analyzed by RT-qPCR and multiplex ELISA before and after stimulation. Results: IL-33 expression in unstimulated BECs and IL-33 protein levels in BALF were reduced after 12 weeks of tezepelumab. Further, IL-33 gene and protein levels decreased in BECs challenged with poly(I:C) after tezepelumab whereas TSLP gene expression remained unaffected. Poly(I:C)-induced IL-4, IL-13, and IL-17A release from BECs was also reduced with tezepelumab whereas IFNβ and IFNλ expression and viral load were unchanged. Conclusion: Blocking TSLP with tezepelumab in vivo in asthma reduced the airway epithelial inflammatory response including IL-33 and T2 cytokines to viral challenge without affecting anti-viral host resistance. Our results suggest that blocking TSLP stabilizes the bronchial epithelial immune response to respiratory viruses.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Lungmedicin och allergi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Respiratory Medicine and Allergy (hsv//eng)
Nyckelord
- airway epithelium
- asthma
- tezepelumab
- virus
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Sverrild, A.
-
Cerps, S.
-
Nieto-Fontarigo, ...
-
Ramu, S.
-
Hvidtfeldt, M.
-
Menzel, M.
-
visa fler...
-
Kearley, J.
-
Griffiths, J. M.
-
Parnes, J. R.
-
Porsbjerg, C.
-
Uller, L.
-
visa färre...
- Om ämnet
-
- MEDICIN OCH HÄLSOVETENSKAP
-
MEDICIN OCH HÄLS ...
-
och Klinisk medicin
-
och Lungmedicin och ...
- Artiklar i publikationen
-
Allergy: Europea ...
- Av lärosätet
-
Lunds universitet